Kilic, N. and Oliveira-Ferrer, L. and Wurmbach, J. H. and Loges, S. and Chalajour, F. and Vahid, S. N. and Weil, Joachim and Fernando, M. and Erguen, S. (2005) Pro-angiogenic signaling by the endothelial presence of CEACAM1. JOURNAL OF BIOLOGICAL CHEMISTRY, 280 (3). pp. 2361-2369. ISSN 0021-9258, 1083-351X
Full text not available from this repository. (Request a copy)Abstract
Here, we demonstrate the expression of carcinoembryonic antigen-related cell adhesion molecule-1 (CEACAM1) in angiogenic sprouts but not in large mother blood vessels within tumor tissue. Correspondingly, only human microvascular endothelial cells involved in in vitro tube formation exhibit CEACAM1. CEACAM1-overexpressing versus CEACAM1-silenced human microvascular endothelial cells were used in migration and tube formation assays. CEACAM1-overexpressing microvascular endothelial cells showed prolonged survival and increased tube formation when they were stimulated with vascular endothelial growth factor ( VEGF), whereas CEACAM1 silencing via small interfering RNA blocks these effects. Gene array and LightCycler analyses show an up-regulation of angiogenic factors such as VEGF, VEGF receptor 2, angiopoietin-1, angiopoietin-2, tie-2, angiogenin, and interleukin-8 but a down-regulation of collagen XVIII/endostatin and Tie-1 in CEACAM1-overexpressing microvascular endothelial cells. Western blot analyses confirm these results for VEGF and endostatin at the protein level. These results suggest that constitutive expression of CEACAM1 in microvascular endothelial cells switches them to an angiogenic phenotype, whereas CEACAM1 silencing apparently abrogates the VEGF-induced morphogenetic effects during capillary formation. Thus, strategies targeting the endothelial up-regulation of CEACAM1 might be promising for antiangiogenic tumor therapy.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CELL-ADHESION MOLECULE; CARCINOEMBRYONIC ANTIGEN FAMILY; BLOOD-VESSEL FORMATION; BILIARY GLYCOPROTEIN; GROWTH-FACTOR; TUMOR ANGIOGENESIS; C-CAM; COLORECTAL CARCINOMAS; PROSTATE-CANCER; VEGF; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 31 May 2021 08:47 |
| Last Modified: | 31 May 2021 08:47 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36581 |
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