da Paula, A. C. and Ramalho, A. S. and Farinha, C. M. and Cheung, J. and Maurisse, R. and Gruenert, Dieter C. and Ousingsawat, J. and Kunzelmann, Karl and Amaral, M. D. (2005) Characterization of novel airway submucosal gland cell models for cystic fibrosis studies. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 15 (6). pp. 251-262. ISSN 1015-8987, 1421-9778
Full text not available from this repository. (Request a copy)Abstract
Cultured airway,epithelial cells are widely used in cystic fibrosis (CF) research as in vitro models that mimic the in vivo manifestations of the disease and help to define a specific cellular phenotype. Recently, a number of in vitro studies have used an airway adenocarcinoma cell line, Calu-3 that expresses submucosal gland cell features and significant levels of the wild-type CFTR mRNA and protein. We further characterized previously described CF tracheobronchial gland cell lines, CFSMEo- and 6CFSMEo- and determined that these cell lines are compound heterozygotes for the F508del and Q2X mutations, produce vestigial amounts of CFTR mRNA, and do not express detectable CFTR protein. Electrophysiologically, both cell lines are characteristically CF in that they lack cAMP-induced Cl- currents. In this study the cell lines are evaluated in the context of their role as the CF correlate to the Calu-3 cells. Together these cell systems provide defined culture systems to study the biology and pathology of CF. These airway epithelial cell lines may also be a useful negative protein control for numerous studies involving gene therapy by cDNA complementation or gene targeting. Copyright (C) 2005 S. Karger AG, Basel.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TRANSMEMBRANE CONDUCTANCE REGULATOR; NASAL EPITHELIAL-CELLS; DEFECTIVE SIMIAN VIRUS-40; CHLORIDE SECRETION; DELTA-F508 CFTR; MESSENGER-RNA; TRANSCRIPT ANALYSIS; EXPRESSION; GENE; LOCALIZATION; CFTR; cystic fibrosis; CF cellular model; epithelial cells; Calu-3; submucosal gland; airway cell line |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Karl Kunzelmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Jun 2021 04:42 |
| Last Modified: | 02 Jun 2021 04:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36652 |
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