Pilsl, Michael and Crucifix, Corinne and Papai, Gabor and Krupp, Ferdinand and Steinbauer, Robert and Griesenbeck, Joachim and Milkereit, Philipp and Tschochner, Herbert and Schultz, Patrick (2016) Structure of the initiation-competent RNA polymerase I and its implication for transcription. Nature Communications, 7: 12126. ISSN 2041-1723,
Full text not available from this repository. (Request a copy)Abstract
Eukaryotic RNA polymerase I (Pol I) is specialized in rRNA gene transcription synthesizing up to 60% of cellular RNA. High level rRNA production relies on efficient binding of initiation factors to the rRNA gene promoter and recruitment of Pol I complexes containing initiation factor Rrn3. Here, we determine the cryo-EM structure of the Pol I-Rrn3 complex at 7.5 angstrom resolution, and compare it with Rrn3-free monomeric and dimeric Pol I. We observe that Rrn3 contacts the Pol I A43/A14 stalk and subunits A190 and AC40, that association re-organizes the Rrn3 interaction interface, thereby preventing Pol I dimerization; and Rrn3-bound and monomeric Pol I differ from the dimeric enzyme in cleft opening, and localization of the A12.2 C-terminus in the active centre. Our findings thus support a dual role for Rrn3 in transcription initiation to stabilize a monomeric initiation competent Pol I and to drive pre-initiation complex formation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SACCHAROMYCES-CEREVISIAE; ANGSTROM RESOLUTION; ELECTRON-MICROSCOPY; ELONGATION COMPLEX; RDNA TRANSCRIPTION; CORE FACTOR; YEAST; ARCHITECTURE; CLEAVAGE; BINDING; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Apr 2019 11:33 |
| Last Modified: | 09 Apr 2019 11:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3667 |
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