Couillard-Despres, Sebastien and Winner, Beate and Schaubeck, Susanne and Aigner, Robert and Vroemen, Maurice and Weidner, Norbert and Bogdahn, Ulrich and Winkler, Juergen and Kuhn, Hans-Georg and Aigner, Ludwig (2005) Doublecortin expression levels in adult brain reflect neurogenesis. EUROPEAN JOURNAL OF NEUROSCIENCE, 21 (1). pp. 1-14. ISSN 0953-816X, 1460-9568
Full text not available from this repository. (Request a copy)Abstract
Progress in the field of neurogenesis is currently limited by the lack of tools enabling fast and quantitative analysis of neurogenesis in the adult brain. Doublecortin (DCX) has recently been used as a marker for neurogenesis. However, it was not clear whether DCX could be used to assess modulations occurring in the rate of neurogenesis in the adult mammalian central nervous system following lesioning or stimulatory factors. Using two paradigms increasing neurogenesis levels (physical activity and epileptic seizures), we demonstrate that quantification of DCX-expressing cells allows for an accurate measurement of modulations in the rate of adult neurogenesis. Importantly, we excluded induction of DCX expression during physiological or reactive gliogenesis and excluded also DCX re-expression during regenerative axonal growth. Our data validate DCX as a reliable and specific marker that reflects levels of adult neurogenesis and its modulation. We demonstrate that DCX is a valuable alternative to techniques currently used to measure the levels of neurogenesis. Importantly, in contrast to conventional techniques, analysis of neurogenesis through the detection of DCX does not require in vivo labelling of proliferating cells, thereby opening new avenues for the study of human neurogenesis under normal and pathological conditions.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NEURAL STEM-CELLS; FIBROBLAST GROWTH FACTOR-2; NEWLY GENERATED NEURONS; LONG-TERM SURVIVAL; SPINAL-CORD-INJURY; RAT DENTATE GYRUS; SUBVENTRICULAR ZONE; OLFACTORY-BULB; HIPPOCAMPAL NEUROGENESIS; ALZHEIMERS-DISEASE; neural stem cells; neurogenesis indicator; neuronal differentiation; neuronal progenitor |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Neurologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Jun 2021 06:48 |
| Last Modified: | 02 Jun 2021 06:48 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36688 |
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