Farkas, Stefan A. and Hornung, M. and Sattler, C. and Steinbauer, M. and Anthuber, M. and Obermeier, F. and Herfarth, H. and Schlitt, H. J. and Geissler, E. K. (2005) Preferential migration of CD62L(+) cells into the appendix in mice with experimental chronic colitis. EUROPEAN SURGICAL RESEARCH, 37 (2). pp. 115-122. ISSN 0014-312X,
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Background: Clinical and experimental studies suggest that appendectomy can protect against development of ulcerative colitis and Crohn's disease. However, how T cells in the appendix affect the development of colitis has not been clarified. Aim: To investigate the in vivo migration and activation of colitis- inducing CD62L(+) cells during development of chronic colitis. Methods: CD62L(+) CD4(+) cells were fluorescently labeled and transferred to severe combined immunodeficient ( SCID) mice to induce colitis. In vivo migration of T cells into the mucosa of the appendix and colon was quantified by in vivo microscopy after 7 weeks. In a second experiment, unlabeled CD62L(+) CD4(+) cells were transferred, reisolated after 7 weeks, and adhesion molecule ( integrin alpha(4)beta(7)) and costimulatory molecule ( CD154) expression was analyzed. Results: Six to eight weeks after CD62L(+) CD4(+) cell transfer, SCID mice developed chronic colitis. In vivo microscopic analysis demonstrated a preferential migration of fluorescence- labeled CD62L(+) CD4(+) cells into the mucosa of the appendix versus the colon. Re- isolation of lamina propria cells from mice with colitis confirmed that CD62L(+) CD4(+) cell migration was significantly enhanced in the appendix, compared to the colon ( 3.5- fold). Furthermore, a higher proportion of CD62L(+) CD4(+) cells re- isolated from the appendix expressed integrin alpha(4)beta(7) and CD154 than from the colon. Conclusion: This study demonstrates the preferential migration of CD62L(+) CD4(+) cells into the appendix as compared to the colon. This migration pattern correlated with upregulation of integrin alpha(4)beta(7) and CD154 ( CD40 ligand) on T cells. Our results suggest an important role of the appendix in the pathogenesis of colitis. Copyright (C) 2005 S. Karger AG, Basel.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INFLAMMATORY-BOWEL-DISEASE; CD4(+) T-CELLS; ADHESION MOLECULE-1 MADCAM-1; ULCERATIVE-COLITIS; SCID MICE; LYMPHOID-TISSUE; CROHNS-DISEASE; APPENDECTOMY; INTEGRIN; CD45RB(HIGH); experimental chronic colitis; in vivo microscopy; appendix; CD62L(+) cell migration; adhesion molecules |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Jun 2021 06:50 |
| Last Modified: | 02 Jun 2021 06:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36689 |
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