Shalguev, V. I. and Kaboev, O. K and Sizova, I. A. and Hagemann, P. and Lanzov, V. A. (2005) Identification of Chlamydomonas reinhardtii Rad51C: Recombinational characteristics. MOLECULAR BIOLOGY, 39 (1). pp. 98-104. ISSN 0026-8933, 1608-3245
Full text not available from this repository. (Request a copy)Abstract
Unicellular green alga Chlamydomonas reinhardtii is a promising model for fundamental and biotechnological research. However, little is known about its system of homologous recombination underlying recombination repair of double-strand breaks. Sequencing of the C reinhardtii nuclear genome has revealed many repeats, which account for a low level of nuclear homologous recombination compared to that of nonhomologous recombination. Analysis of C reinhardtii EST and genomic libraries made it possible to reconstruct and clone the RAD51C cDNA. In this work, this cDNA was expressed, the protein product was purified, and its main biochemical activities were studied. It was shown that Rad51C of lower eukaryote C. reinhardtii is a typical member of the subfamily of higher eukaryotic Rad51-like recombination proteins.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DNA-REPAIR PROTEINS; RECA PROTEIN; COMPLEX-FORMATION; ESCHERICHIA-COLI; PARALOGS; PURIFICATION; Chlamydomonas reinhardtii; Rad51C; homologous recombination; biochemical activities of DNA transferases |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Jun 2021 10:35 |
| Last Modified: | 08 Jun 2021 10:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36773 |
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