Tang, T. H. and Polacek, N. and Zywicki, M. and Huber, Harald and Brugger, K. and Garrett, R. and Bachellerie, J. P. and Huettenhofer, Alexander (2005) Identification of novel non-coding RNAs as potential antisense regulators in the archaeon Sulfolobus solfataricus. MOLECULAR MICROBIOLOGY, 55 (2). pp. 469-481. ISSN 0950-382X, 1365-2958
Full text not available from this repository. (Request a copy)Abstract
By generating a specialized cDNA library from the archaeon Sulfolobus solfataricus, we have identified 57 novel small non-coding RNA (ncRNA) candidates and confirmed their expression by Northern blot analysis. The majority was found to belong to one of two classes, either antisense or antisense-box RNAs, where the latter only exhibit partial complementarity to RNA targets. The most prominent group of antisense RNAs is transcribed in the opposite orientation to the transposase genes, encoded by insertion elements (transposons). Thus, these antisense RNAs may regulate transposition of insertion elements by inhibiting expression of the transposase mRNA. Surprisingly, the class of antisense RNAs also contained RNAs complementary to tRNAs or sRNAs (small-nucleolar-like RNAs). For the antisense-box ncRNAs, the majority could be assigned to the class of C/D sRNAs, which specify 2'-O-methylation sites on rRNAs or tRNAs. Five C/D sRNAs of this group are predicted to target methylation at six sites in 13 different tRNAs, thus pointing to the widespread role of these sRNA species in tRNA modification in Archaea. Another group of antisense-box RNAs, lacking typical C/D sRNA motifs, was predicted to target the 3'-untranslated regions of certain mRNAs. Furthermore, one of the ncRNAs that does not show antisense elements is transcribed from a repeat unit of a cluster of small regularly spaced repeats in S. solfataricus which is potentially involved in replicon partitioning. In conclusion, this is the first report of stably expressed antisense RNAs in an archaeal species and it raises the prospect that antisense-based mechanisms are also used widely in Archaea to regulate gene expression.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NON-MESSENGER RNAS; SMALL NUCLEOLAR RNAS; REGULARLY SPACED REPEATS; SNORNA GENE CLUSTERS; ESCHERICHIA-COLI; ARABIDOPSIS-THALIANA; MOBILE ELEMENTS; COMPLETE GENOME; RNOMICS; CANDIDATES; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Jun 2021 10:37 |
| Last Modified: | 08 Jun 2021 10:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36775 |
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