Bodzioch, M. and Dembinska-Kiec, A. and Hartwich, J. and Lapicka-Bodzioch, K. and Banas, A. and Polus, A. and Grzybowska, J. and Wybranska, I. and Dulinska, J. and Gil, D. and Laidler, P. and Placha, W. and Zawada, M. and Balana-Nowak, A. and Sacha, T. and Kiec-Wilk, B. and Skotnicki, A. and Moehle, C. and Langmann, T. and Schmitz, Gerd (2005) The microarray expression analysis identifies BAX as a mediator of beta-carotene effects on apoptosis. NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 51 (2). pp. 226-235. ISSN 0163-5581, 1532-7914
Full text not available from this repository. (Request a copy)Abstract
beta-Carotene is a ubiquitous compound rich in foods. However, there are conflicting reports regarding its role in carcinogenesis. We performed a microarray expression analysis in normal [human umbilical vein endothelial cells (HUVECs)] and neoplastic (melanoma A375 and myelomonocytic leukemia U937) actively proliferating cells and found evidence that beta-carotene stimulated vital cellular functions in the former and suppressed them in the latter. These differential effects correlated with the expression of the proapoptotic BCL2-associated X protein (BAX), which was downregulated in HUVECs and upregulated in the two neoplastic cell lines. The quantitative expression analysis using real-time polymerase chain reaction largely confirmed the inhibition of B-cell CLL/lymphoma 2 (BCL2) pathway-mediated apoptosis in HUVECs and its activation in melanoma and leukemic cells. The assays for apoptosis, detecting DNA breaks and caspase activation, showed consistent proapoptotic and antiapoptotic effects in U937 and HUVEC lines, respectively. However, beta-carotene-induced expression changes of BAX and other BCL2 pathway genes did not lead to the predicted induction of apoptosis in the A375 cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CANCER PREVENTION; REDOX MECHANISM; RETINOIC ACID; LUNG-CANCER; CELLS; VEGETABLES; RESISTANCE; RECEPTORS; LEUKEMIA; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Jun 2021 13:30 |
| Last Modified: | 08 Jun 2021 13:30 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36785 |
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