Yegorova, Alla and Karasyov, Alexander and Duerkop, Axel and Ukrainets, Igor and Antonovich, Valery (2005) New luminescent terbium complex for the determination of DNA. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 61 (1-2). pp. 109-116. ISSN 1386-1425,
Full text not available from this repository. (Request a copy)Abstract
New terbium complexes of derivatives of 2-oxo-4-hydroxy-quinoline-3-carboxylic acid are reported, which are highly luminescent, water soluble and do not require luminescence enhancers. The triplet-state energy levels of the ligands, the relative quantum yields (QYs) and the excitation maxima of the respective terbium chelates were determined. The large luminescence enhancement of one of these complexes by nucleic acids was investigated and a mechanism of its interaction with DNA is proposed. The optimal conditions for determination of DNA are equal concentrations of Tb3+ and ligand R-1 (C = 1 x 10(-6) M), pH 9.0. Under optimal conditions the luminescence intensity (RI) is proportional to the concentration of fish sperm DNA (fsDNA) or calf thymus DNA (ctDNA), respectively, within the range of 0.05-1.5 mug ml(-1). The detection limits were 10 ng ml(-1) for fsDNA and 12 ng ml(-1) for ctDNA. (C) 2004 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NUCLEIC-ACIDS; FLUOROMETRIC METHOD; FLUORESCENCE; PROBE; RNA; ASSAY; terbium complex; nucleic acids; luminescence; intercalator |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Jun 2021 09:17 |
| Last Modified: | 21 Jun 2021 09:17 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36830 |
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