Guertler, Ulrich and Fuchs, Peter and Stangelmayer, Achim and Bernhardt, Gunther and Buschauer, Armin and Spruss, Thilo (2004) Construction and validation of a microprocessor controlled extracorporal circuit in rats for the optimization of isolated limb perfusion. ARCHIV DER PHARMAZIE, 337 (12). pp. 672-681. ISSN 0365-6233, 1521-4184
Full text not available from this repository. (Request a copy)Abstract
Although a few experimental approaches to isolated limb perfusion (ILP) are described in the literature, none of these animal models mimics the clinical perfusion techniques adequately to improve the technique of ILP on the basis of valid preclinical data. Therefore, we developed an ILP setup in rats allowing online monitoring of essential perfusion parameters such as temperature (in perfusate, various tissues, and rectum), pH (perfusate), perfusion pressure, and O-2 concentration (in perfusate, tissue), by a tailor-made data acquisition system. This setup permits close supervision of vital parameters during ILP. Various interdependencies, concerning the flow rate and the pressure of perfusate as well as tissue oxygenation were registered. For the measurement of pO(2) values in the perfusate and in different regions of the perfused hind limb, a novel type of microoptode based on quenching of a fluorescent dye was devised. Stable normothermic (37degreesC) perfusion conditions were maintained at a constant perfusion pressure in the range of 40-60 mm Hg by administration of the spasmolytic moxaverine (0.5 mg/mL of perfusate as initial dose) at a perfusate flow rate of 0.5 mL/min for 60 min. At the end of an ILP, there were no signs of tissue damage, neither concerning laboratory data (K+, myoglobin, creatine kinase, lactic dehydrogenase) nor histopathological criteria. The reported ILP model is not only well suited to investigate the effects of hyperthermia but also to assess the efficacy of new antineoplastic approaches, when nude rats, bearing human tumours in the hind limbs, are used.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NECROSIS-FACTOR-ALPHA; REGIONAL PERFUSION; MALIGNANT-MELANOMA; RECURRENT MELANOMA; SKELETAL-MUSCLE; ANIMAL-MODEL; HIND-LIMB; TNF-ALPHA; NUDE RATS; MELPHALAN; limb perfusion; rat; online monitoring; oxygen-microoptodes; perfusion pressure |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Alumni or Retired Professors > Pharmaceutical/Medicinal Chemistry II (Prof. Buschauer) Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Jun 2021 09:12 |
| Last Modified: | 21 Jun 2021 09:12 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36881 |
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