Klein, Silvan M. and Vykoukal, Jody and Li, De-Pei and Pan, Hui-Lin and Zeitler, Katharina and Alt, Eckhard and Geis, Sebastian and Felthaus, Oliver and Prantl, Lukas (2016) Peripheral Motor and Sensory Nerve Conduction following Transplantation of Undifferentiated Autologous Adipose Tissue-Derived Stem Cells in a Biodegradable US Food and Drug Administration-Approved Nerve Conduit. PLASTIC AND RECONSTRUCTIVE SURGERY, 138 (1). pp. 132-139. ISSN 0032-1052, 1529-4242
Full text not available from this repository. (Request a copy)Abstract
Background: Conduits preseeded with either Schwann cells or stem cells differentiated into Schwann cells demonstrated promising results for the outcome of nerve regeneration in nerve defects. Methods: The concept of this trial combines nerve repair by means of a commercially available nerve guidance conduit and preseeding with autologous, undifferentiated, adipose tissue-derived stem cells. Adipose tissue-derived stem cells were harvested from rats and subsequently seeded onto a U.S. Food and Drug Administration-approved type I collagen conduit. Sciatic nerve gaps 10 mm in length were created, and nerve repair was performed by the transplantation of either conduits preseeded with autologous adipose tissue-derived stem cells or acellular (control group) conduits. After 6 months, the motor and sensory nerve conduction velocity were assessed. Nerves were removed and examined by hematoxylin and eosin, van Gieson, and immunohistochemistry (S100 protein) staining for the quality of axonal regeneration. Results: Nerve gaps treated with adipose tissue-derived stem cells showed superior nerve regeneration, reflected by higher motor and sensory nerve conduction velocity values. The motor and sensory nerve conduction velocity were significantly greater in nerves treated with conduits preseeded with adipose tissue-derived stem cells than in nerves treated with conduits alone (p < 0.05). Increased S100 immunoreactivity was detected for the adipose tissue-derived stem cell group. In this group, axon arrangement inside the conduits was more organized. Conclusions: Transplantation of adipose tissue-derived stem cells significantly improves motor and sensory nerve conduction velocity in peripheral nerve gaps. Preseeded conduits showed a more organized axon arrangement inside the conduit in comparison with nerve conduits alone. The approach used here could readily be translated into a clinical therapy.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GROWTH-FACTOR RECEPTOR; MARROW STROMAL CELLS; SCHWANN-CELLS; IN-VITRO; SCIATIC-NERVE; NEUROTROPHIC FACTOR; MALIGNANT-TRANSFORMATION; ADULT-RAT; REGENERATION; INJURY; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Apr 2019 11:41 |
| Last Modified: | 09 Apr 2019 11:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3689 |
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