Malinska, Katerina and Malinsky, Jan and Opekarova, Miroslava and Tanner, Widmar (2004) Distribution of Can1p into stable domains reflects lateral protein segregation within the plasma membrane of living S. cerevisiae cells. JOURNAL OF CELL SCIENCE, 117 (25). pp. 6031-6041. ISSN 0021-9533, 1477-9137
Full text not available from this repository. (Request a copy)Abstract
Recently, lipid-raft-based subdomains within the plasma membrane of living Saccharomyces cerevisiae cells were visualized using green fluorescent protein fusions, and non-overlapping subdomains containing either Pma1p or Can1p were distinguished. In this study, the long-term stability of the subdomains was investigated. Experiments with latrunculin A and nocodazole ruled out the involvement of cytoskeletal components in the stabilization of the subdomains. Also a putative role of the cell wall was excluded, because protoplasting of the cells changed neither the pattern nor the stability of the subdomains. By contrast, the expected inner dynamics of the membrane subdomains was documented by FRAP experiments. Finally, two other proteins were localized within the frame of the Can1p/Pma1p plasma-membrane partition. We show that Fur4p (another H+ symporter) and Sur7p (a protein of unknown function) occupy the Can1p subdomain.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SACCHAROMYCES-CEREVISIAE; LIPID RAFTS; TARGETING PROTEINS; STRESS-RESPONSE; YEAST; DELIVERY; SURFACE; DIFFUSION; CAVEOLAE; PERMEASE; lipid rafts; CAN1; FUR4; SUR7; PMA1 |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Jun 2021 10:43 |
| Last Modified: | 21 Jun 2021 10:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36909 |
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