Lottner, Christian and Knuechel, Ruth and Bernhardt, Guenther and Brunner, Henri (2004) Distribution and subcellular localization of a water-soluble hematoporphyrin-platinum(II) complex in human bladder cancer cells. CANCER LETTERS, 215 (2). pp. 167-177. ISSN 0304-3835,
Full text not available from this repository. (Request a copy)Abstract
The water-soluble porphyrin-platinum complex diammine {7,12-bis[1-(polyethyleneglycol-750-monomethylether1-yl)ethyl]-3,8,13,17-tetramethylporphyrin-2,18-dipropionato} platinum(II) (PEG-HPPt) was studied with respect to cellular accumulation, subcellular localization, behavior in 3D-cell aggregates and degree of DNA platination on the low-differentiated J82 cells, a model of invasive bladder cancer, and UROtsa, a normal urothelial cell line. Accumulation studies with 2D and spheroid cell cultures revealed that the concentration of PEG-HPPt was 1.7-times higher in J82 cancer cells than in UROtsa cells. Despite its high molecular weight, penetration of PEG-HPPt was not restricted to the peripheral cells of the spheroids. Fluorescence microscopic analysis showed that PEG-HPPt was localized in essential cellular targets of photodynamic therapy. DNA platination in J82 and UROtsa cells was higher by PEG-HPPt than by cisplatin, whereas there was no significant difference between the two cell lines. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DIAQUA(1,2-DIPHENYLETHYLENEDIAMINE) PLATINUM(II) SULFATES; INDUCED PROTOPORPHYRIN-IX; DNA ADDUCT FORMATION; PHOTODYNAMIC THERAPY; CISPLATIN RESISTANCE; UROTHELIAL CELLS; TUMOR; REPAIR; LINES; CARCINOMA; porphyrin-platinum complex; bladder cancer; photodynamic therapy; chemotherapy; selective accumulation |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine 600 Technology > 615 Pharmacy |
| Divisions: | Medicine > Lehrstuhl für Pathologie Chemistry and Pharmacy > Institute of Pharmacy Chemistry and Pharmacy > Institut für Organische Chemie > Lehrstuhl Prof. Dr. Burkhard König |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Jun 2021 12:48 |
| Last Modified: | 21 Jun 2021 12:48 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36944 |
Actions (login required)
 |
View Item |
