Bollheimer, L. Cornelius and Landauer, Heike C. and Troll, Stephanie and Schweimer, Joachim and Wrede, Christian E. and Schoelmerich, Juergen and Buettner, Roland (2004) Stimulatory short-term effects of free fatty acids on glucagon secretion at low to normal glucose concentrations. METABOLISM-CLINICAL AND EXPERIMENTAL, 53 (11). pp. 1443-1448. ISSN 0026-0495,
Full text not available from this repository.Abstract
While free fatty acids (FFA) are well known as insulin secretagogues, their effects on pancreatic a cells have been mostly neglected. In the present study we therefore systematically analyzed the glucagon metabolism of rat pancreatic islets under the influence of FFA. Primary islets were incubated in the presence or absence of 200 mumol/L albumin-complexed palmitate or oleate at 2.8 mmol/L versus 16.7 mmol/L glucose and glucagon secretion was monitored over 8 hours. In addition to these time-course experiments, dose dependency of palmitate-induced effects was tested by a 2-hour incubation with 50 to 300 mumol/L albumin-complexed palmitate at 2.8 mmol/L and 5.6 mmol/L glucose. Apart from glucagon secretion, intracellular immunoreactive glucagon and cellular preproglucagon-mRNA (PPG-mRNA) content were determined from the remaining cell lysates. FFA, especially palmitate, induced a significant and dose-dependent increase of glucagon secretion (in average 2-fold above control) during the first 120 minutes of incubation at low to normal glucose (2.8 and 5.6 mmol/L). There was no significant glucagonotropic effect of FFA at concomitant 16.7 mmol/L glucose. Intracellular glucagon as well as cellular PPG-mRNA content were found to be dose-dependently diminished by palmitate when compared with untreated controls at 5.6 mmol/L glucose. The present analysis therefore points to a new role for FFA as a nutritient secretagogue and a modulator of a-cellular glucagon metabolism. (C) 2004 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RAT PANCREATIC-ISLETS; INSULIN-SECRETION; PROINSULIN BIOSYNTHESIS; CELLS; METABOLISM; LANGERHANS; RESISTANCE; PHYSIOLOGY; EXPOSURE; INCREASE; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Jun 2021 08:08 |
| Last Modified: | 23 Jun 2021 08:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37009 |
Actions (login required)
 |
View Item |
