Kzhyshkowska, Julia and Kremmer, Elisabeth and Hofmann, Markus and Wolf, Hans and Dobner, Thomas (2004) Protein arginine methylation during lytic adenovirus infection. BIOCHEMICAL JOURNAL, 383. pp. 259-265. ISSN 0264-6021,
Full text not available from this repository. (Request a copy)Abstract
Arginine methylation of proteins affects major processes in the cell, including transcriptional regulation, mRNA metabolism, signal transduction and protein sorting. Arginine methylation of Ad (adenovirus) E1B 55-kDa-associated protein E1B-AP5 was recently described by us [Kzhyshkowska, Schutt, Liss, Kremmer, Stauber, Wolf and Dobner (200 1) Biochem. J. 358, 305-314]. In this first example of protein arginine methylation analysis in Ad-infected cells, we investigated methylation of the E1B-AP5 and the viral L4-100 kDa protein. We demonstrate that E1B-AP5 methylation is enhanced during the course of infection in a cell-type-specific manner. We also show that L4-100 kDa is efficiently methylated in Ad-infected cells. L4-100 kDa formed complex with methyltransferase in vivo during productive infection, and can be methylated by HRMT1L2 (human protein arginine methyltransferase 1) in vitro. Comparative analysis of E1B-AP5 and L4-100 kDa protein methylation in Ad-infected HeLa, MCF-7 and H1299 cells revealed that the profile of protein arginine methylation correlates with the efficiency of Ad proteins production. Our results suggest that protein arginine methylation is an important host-cell function required for efficient Ad replication.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NUCLEAR RIBONUCLEOPROTEIN E1B-AP5; L4 100-KILODALTON PROTEIN; RNA-BINDING ACTIVITY; PRE-MESSENGER-RNA; NUCLEOCYTOPLASMIC TRANSPORT; MONOCLONAL-ANTIBODIES; E4ORF4 PROTEIN; POLYMERASE-II; RGG BOX; TRANSLATION; adenovirus; E1B-AP5; L4-100 kDa; protein arginine methyltransferase; S-adenosyl-L-methionine |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Jun 2021 04:40 |
| Last Modified: | 28 Jun 2021 04:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37079 |
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