Fischbach, Claudia and Spruss, Thilo and Weiser, Barbara and Neubauer, Markus and Becker, Christian and Hacker, Michael and Goepferich, Achim and Blunk, Torsten (2004) Generation of mature fat pads in vitro and in vivo utilizing 3-D long-term culture of 3T3-L1 preadipocytes. EXPERIMENTAL CELL RESEARCH, 300 (1). pp. 54-64. ISSN 0014-4827, 1090-2422
Full text not available from this repository. (Request a copy)Abstract
Tissue-inherent factors such as cell-cell and cell-extracellular matrix interactions are regarded to exert a potentially large impact on adipogenesis as well as on secretory functions of adipose tissue. However, an appropriate 3-D adipogenesis model useful for addressing such interactions is still lacking. In this study, using tissue-engineering techniques, we demonstrate for the first time the development of coherent fat pads consisting of unilocular signet-ring cells in vitro. The constructs were generated by differentiating 3T3-L1 preadipocytes on 3-D polymeric scaffolds for either 9, 21, or 35 days in vitro. Only long-term culture yielded uniform tissues histologically comparable to native fat. Light and scanning electron microscopy provided direct evidence of 3-D tissue coherence and cell-cell contact in a tissue context, which was in strong contrast to conventional 2-D monolayer culture. Further differences between the two culture systems included enhanced secretion of leptin in 3-D tissue culture and differences in laminin expression (mRNA and protein level). Increase of triglyceride content over culture time and mRNA expression of other adipocyte genes, such as PPARgamma and Glut-4, were found to be similar. Implantation of long-term differentiated tissue constructs in nude mice resulted in further development and maintenance of fat pads. The presented model system is suggested to contribute to a better understanding of adipose tissue development and function facilitating studies on tissue-inherent interactions in vitro and in vivo. (C) 2004 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ADIPOSE CONVERSION; CELL-LINE; DIFFERENTIATION; ADIPOGENESIS; ADIPOCYTE; EXPRESSION; SCAFFOLDS; adipocyte; adipogenesis; adipose tissue engineering; extracellular matrix; fat; leptin; scaffold; unilocular; 3-D cell culture; 3T3-L1 |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Jun 2021 04:43 |
| Last Modified: | 28 Jun 2021 04:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37080 |
Actions (login required)
 |
View Item |
