Framme, Carsten and Schuele, Georg and Birngruber, Reginald and Roider, Johann and Schuett, Florian and Kopitz, Jürgen and Holz, Frank and Brinkmann, Ralf (2004) Temperature dependent fluorescence of A2-E, the main fluorescent lipofuscin component in the RPE. CURRENT EYE RESEARCH, 29 (4-5). pp. 287-291. ISSN 0271-3683,
Full text not available from this repository. (Request a copy)Abstract
Purpose. A2-E is the dominant fluorophore of lipofuscin in the retinal pigment epithelium. In an in-vitro setup, we determined the temperature-dependent changes of the A2-E fluorescence with the aim of also assessing, the potential value of such measurements for determining retinal temperature by autofluorescence measurements during laser treatment. Methods. A2-E was biosynthesized and diluted in Dimethyl Sulfoxide (DMSO) to 1 muM. Fluorescence measurements were performed with a photospectrorneter under various temperatures ranging from 20degreesC to 75degreesC. Auto fluorescence was excited at 467 nm, and emission was detected around 632 nm. Results. A2-E fluorescence intensity showed a linear decrease concomitant with temperature increment. At 75degreesC. the fluorescence intensity decreased by 43% compared to at 20degreesC. Fluorescence intensity was completely reversible dependent on the temperature, which cannot be explained by thermal A2-E alteration. Conclusions. If the A2-E temperature-dependent fluorescence in-vitro is transferable to human fundus autofluorescence, then it may be possible to apply an autofluorescence-based online detection device for noninvasive determination of fundus temperature during in vivo laser treatment. This is of clinical relevance, especially for the application of photodynamic therapy (PDT) and transpupillary thermotherpy (TTT).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RETINAL-PIGMENT EPITHELIUM; SUBFOVEAL CHOROIDAL NEOVASCULARIZATION; TRANSPUPILLARY THERMOTHERAPY; FUNDUS AUTOFLUORESCENCE; MACULAR DEGENERATION; OCULAR FUNDUS; CELLS; A2-E; fundus autofluorescence; spectral analysis; fundus laser treatment; lipofuscin; transpupillary; thermotherpy; TTT |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Augenheilkunde |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Jun 2021 06:53 |
| Last Modified: | 28 Jun 2021 06:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37106 |
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