Staudenmaier, Rainer and Hoang, T. Nguyen and Kleinsasser, Norbert and Schurr, Christian and Froelich, Katrin and Wenzel, Magdalene M. and Aigner, Joachim (2004) Flap prefabrication and prelamination with tissue-engineered cartilage. JOURNAL OF RECONSTRUCTIVE MICROSURGERY, 20 (7). pp. 555-564. ISSN 0743-684X,
Full text not available from this repository.Abstract
In reconstructive surgery, the integration of tissue-engineered cartilage in a prefabricated free flap may make it possible to generate flaps combining a variety of tissue components, to meet the special requirements of particular defects. One aim of the present study was to investigate prefabrication of a microvascular free flap by implanting a vessel loop under a skin flap in a rabbit model. A second aim was to report on the authors' preliminary experiences in prelaminating prefabricated flaps with autologous tissue-engineered cartilage, in terms of matrix development, inflammatory reaction, and host-tissue interaction. The flap was prefabricated by implanting a vessel loop under a random-pattern abdominal skin flap. The tissue-engineered cartilage constructs were made by isolating chondrocytes from auricular biopsies. Following a period of amplification, the cells were seeded onto a non-woven scaffold made of a hyaluronic-acid derivative and cultivated for 2 weeks. One cell-biomaterial construct was placed beneath the prefabicated flap, and two additional constructs were placed subcutaneously and intramuscularly. In addition, a biomaterial sample without cells was placed subcutaneously to provide a control. All implanted specimens were left in position for 6 or 12 weeks. Neovascularization in the prefabricated flap and biomaterial construct was analyzed by angiography. After explantation, the specimens were examined by histologic and immunohistochemical methods. The prefabricated flaps showed a well-developed network of blood vessels between the implanted vessel loop and the original random-pattern blood supply. The tissue-engineered constructs remained stable in size and showed signs of tissue similar to hyaline cartilage, as evidenced by the expression of cartilage-specific collagen type II and proteoglycans. No inflammatory reactions were observed. The physiologic environment of the autologous rabbit model provided favorable conditions for matrix deposition and maturation of the cell-biomaterial constructs. These initial results demonstrated the potential of prefabricating an axial perfused flap, combined with tissue-engineered cartilage, thus creating functionally competent tissue components for reconstructive surgery with minimal donor-site morbidity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SKIN FLAPS; IN-VITRO; ARTERIOVENOUS PEDICLE; VENOUS FLAPS; BONE-MATRIX; RAT MODEL; RECONSTRUCTION; COLLAGEN; PERICHONDRIUM; CHONDROCYTES; prefabrication; prelamination; tissue engineering |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Hals-Nasen-Ohren-Heilkunde |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Jun 2021 06:54 |
| Last Modified: | 28 Jun 2021 06:54 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37143 |
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