Lindner, Petra and Molz, Ramona and Yacoub-George, Erwin and Duerkop, Axel and Wolf, Hans (2004) Development of a highly sensitive inhibition immunoassay for microcystin-LR. ANALYTICA CHIMICA ACTA, 521 (1). pp. 37-44. ISSN 0003-2670,
Full text not available from this repository. (Request a copy)Abstract
The development of a rapid one-step antigen-immobilized inhibition ELBA for microcystin-LR is described. For microplate coating a microcystin-biotin conjugate was synthesized. Using the commercially available monoclonal antibody MC10E7 in our newly established assay, IC50 values of 0.045 mug l(-1) have been achieved. The detection limit for microcystin-LR was 4 ng l(-1). Considering the guidelines proposed by the world health organization (WHO) for microcystin-LR in drinking water (1 mug l(-1)) the sensitivity of our test is more than sufficient. The period of assay processing could successfully be shortened to about 3 h without any loss in sensitivity. The suitability of the newly developed assay was evaluated with microcystin-LR spiked environmental water samples. Recovery rates for microcystin-LR between 60 and 165% were obtained in the linear range of the test format. The antigen-immobilized test format provides a highly reproducible, easy, and fast to perform detection system for microcystin allowing an internal retrospective quality control of the assay. (C) 2004 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LINKED-IMMUNOSORBENT-ASSAY; CYANOBACTERIA; WATER; NODULARINS; BRAZIL; BLOOMS; CHROMATOGRAPHY; CYANOTOXINS; ANTIBODIES; STRAINS; microcystin; inhibition immunoassay; ELISA; cyanobacteria |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Jun 2021 10:10 |
| Last Modified: | 28 Jun 2021 10:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37194 |
Actions (login required)
 |
View Item |
