Wang, Hao-Ven and Vaupel, Kristina and Buettner, Reinhard and Bosserhoff, Anja-Katrin and Moser, Markus (2004) Identification and embryonic expression of a new AP-2 transcription factor, AP-2 epsilon. DEVELOPMENTAL DYNAMICS, 231 (1). pp. 128-135. ISSN 1058-8388, 1097-0177
Full text not available from this repository. (Request a copy)Abstract
AP-2 proteins comprise a family of highly related transcription factors, which are expressed during mouse embryogenesis in a variety of ectodermal, neuroectodermal, and mesenchymal tissues. AP-2 transcription factors were shown to be involved in morphogenesis of craniofacial, urogenital, neural crest-derived, and placental tissues. By means of a partial cDNA fragment identified during an expressed sequence tag search for AP-2 genes, we identified a fifth, previously unknown AP-2-related gene, AP-2epsilon. AP-2epsilon encodes an open reading frame of 434 amino acids, which reveals the typical modular structure of AP-2 transcription factors with highly conserved C-terminal DNA binding and dimerization domains. Although the N-terminally localized activation domain is less homologous, position and identity of amino acids essential for transcriptional transactivation are conserved. Reverse transcriptase-polymerase chain reaction analyses of murine embryos revealed AP-2epsilon expression from gestational stage embryonic day 7.5 throughout all later embryonic stages until birth. Whole-mount in situ hybridization using a specific AP-2epsilon cDNA fragment demonstrated that during embryogenesis, expression of AP-2epsilon is mainly restricted to neural tissue, especially the midbrain, hindbrain, and olfactory bulb. This expression pattern was confirmed by immunohistochemistry with an AP-2epsilon-Specific antiserum. By using this antiserum, we could further localize AP-2e expression in a hypothalamic nucleus and the neuroepithelium of the vomeronasal organ, suggesting an important function of AP-2epsilon for the development of the olfactory system. (C) 2004 Wiley-Liss, Inc.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PATENT DUCTUS-ARTERIOSUS; DNA-BINDING; MOUSE EMBRYOGENESIS; DROSOPHILA HOMOLOG; FACTOR AP-2-BETA; CHAR-SYNDROME; CLONING; MICE; AP-2-GAMMA; AP-2-ALPHA; AP-2; transcription factor; mouse embryogenesis; gene expression |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Jun 2021 13:02 |
| Last Modified: | 28 Jun 2021 13:02 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37227 |
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