Weiss, Thomas S. and Herfarth, Hans and Obermeier, F. and Ouart, J. and Vogl, D. and Schoelmerich, Juergen and Jauch, Karl-Walter and Rogler, Gerhard (2004) Intracellular polyamine levels of intestinal epithelial cells in inflammatory bowel disease. INFLAMMATORY BOWEL DISEASES, 10 (5). pp. 529-535. ISSN 1078-0998,
Full text not available from this repository. (Request a copy)Abstract
Polyamines and their acetylated derivatives are a prerequisite for cellular metabolism and considered to be essential for proliferation and differentiation of the rapidly renewing intestinal mucosa. However, their role during mucosal inflammation is less clear. Polyamine concentrations were determined in isolated colonic epithelial cells (CECs) from endoscopic biopsies from 26 patients with inflammatory bowel disease (IBD) and 40 controls as well as colon samples from mice with and without acute or chronic dextran sodium sulfate (DSS)-induced colitis. In patients with ulcerative colitis, CEC spermidine and N-8-acetylspermidine levels were significantly enhanced and spermine levels were reduced compared with healthy controls. A correlation of polyamine levels of patients with IBD with their corresponding inflammatory index revealed that increased concentrations of spermidine, N-8-acetylspermidine, and N-1-acetylspermine were found in CECs from the most severe inflamed mucosal areas. Using acute and chronic DSS colitis as a model of mucosal inflammation. we found enhanced levels of spermidine and spermine in acute colitis. whereas in chronic inflammation, CEC spermine concentrations were decreased. Our data indicate a lack of the anti-inflammatory polyamine spermine in severe ulcerative colitis and chronic DSS colitis, which may aggravate the disease. Increased spermidine and N-8-acetylspermidine levels reflect increased uptake and metabolism likely due to accelerated proliferation and regeneration of CECS.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NITRIC-OXIDE SYNTHASE; ULCERATIVE-COLITIS; ORNITHINE-DECARBOXYLASE; DIETARY POLYAMINES; COLONIC-MUCOSA; CROHNS-DISEASE; INHIBITION; SPERMINE; GROWTH; RAT; colitis; colonic epithelial cells; Crohn's disease; inflammatory bowel disease; polyamine |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Jun 2021 12:32 |
| Last Modified: | 28 Jun 2021 12:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37245 |
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