Schaeferling, Michael and Wu, Meng and Wolfbeis, Otto S. (2004) Time-resolved fluorescent imaging of glucose. JOURNAL OF FLUORESCENCE, 14 (5). pp. 561-568. ISSN 1053-0509,
Full text not available from this repository. (Request a copy)Abstract
A method for the fluorescent imaging of glucose is described that is based on the detection of enzymatically produced hydrogen peroxide, using the europium(III) tetracycline complex as the fluorescent probe incorporated into a hydrophilic polymer layer. Coadsorption of glucose oxidase (GOx) makes these sensor layers respond to the hydrogen peroxide produced by the GOx-assisted oxidation of glucose. The hydrogel layers are integrated into a 96-microwell plate for a parallel and simultaneous detection of various samples. Glucose is visualized by means of time resolved luminescence lifetime imaging. Unlike in previous methods, the determination of H2O2 does not require the addition of peroxidase or a catalyst to form a fluorescent product. The lifetime-based images obtained are compared with conventional fluorescence intensity-based methods with respect to sensitivity and the dynamic range of the sensor layer. The main advantages provided by this sensing scheme for H2O2 include reversibility, applicability at neutral pH, and the straightforwardness of the transducer system and the imaging device.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HYDROGEN-PEROXIDE; OPTICAL SENSORS; ENERGY-TRANSFER; OXYGEN SENSORS; IN-VIVO; BIOSENSOR; PROBE; PH; OPTRODE; IMAGES; optical sensor; time-resolved fluorescence imaging; europium tetracycline; glucose sensor |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Jun 2021 06:32 |
| Last Modified: | 29 Jun 2021 06:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37272 |
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