Koehler, Astrid and Bataille, Frauke and Schmid, Cornelia and Ruemmele, Petra and Waldeck, Annette and Blaszyk, Hagen and Hartmann, Arndt and Hofstaedter, Ferdinand and Dietmaier, Wolfgang (2004) Gene expression profiling of colorectal cancer and metastases divides tumours according to their clinicopathological stage. JOURNAL OF PATHOLOGY, 204 (1). pp. 65-74. ISSN 0022-3417, 1096-9896
Full text not available from this repository. (Request a copy)Abstract
Gene expression profiling of matched colorectal carcinomas and metastases could reveal key molecular events involved in tumour progression and metastasis. Expression profiles have been created from 25 colorectal carcinomas (CRCs, pT1-4), corresponding normal colonic mucosa, and 14 liver metastases using cDNA arrays containing 1176 cancer-related genes (Clontech). Hierarchical clustering clearly distinguished carcinomas from non-cancerous tissues, separated tumours into high-stage (pT4 and extensive lymph node or distant metastases) and low-stage (less than or equal topT3) groups, and correlated with the histopathological classification in 87% (33/38 cases). Most primary tumours and matched liver metastases clustered on terminal branches of the dendrogram. Statistical analysis (Mann-Whitney U-test) revealed 40 tumour-specific genes (29 up-regulated, 11 down-regulated) which allowed identification of malignant tissue samples by cluster analysis. A specific expression signature in matching metastases was not found, but a set of 23 classifier genes with statistically significant expression patterns in high- and low-stage tumours was identified. These genes may represent important targets in colorectal carcinogenesis and might provide useful clinicopathological tools in the management of colorectal cancer. Copyright (C) 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley Sons, Ltd.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MICROSATELLITE INSTABILITY; MOLECULAR CLASSIFICATION; OLIGONUCLEOTIDE ARRAYS; TRANSCRIPTION FACTOR; LIVER METASTASES; CDNA MICROARRAY; DNA MICROARRAYS; POOR-PROGNOSIS; BREAST-CANCER; CARCINOMA; cDNA array; colorectal cancer; metastasis; gene expression profiling; cluster analysis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Hals-Nasen-Ohren-Heilkunde Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Jun 2021 06:44 |
| Last Modified: | 29 Jun 2021 06:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37278 |
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