Hidaka, Sumi and Koenecke, Vera and Osten, Larissa and Witzgall, Ralph (2004) PIGEA-14, a novel coiled-coil protein affecting the intracellular distribution of polycystin-2. JOURNAL OF BIOLOGICAL CHEMISTRY, 279 (33). pp. 35009-35016. ISSN 0021-9258, 1083-351X
Full text not available from this repository. (Request a copy)Abstract
Employing a yeast two-hybrid screen with the COOH terminus of polycystin-2, one of the proteins mutated in patients with polycystic kidney disease, we were able to isolate a novel protein that we call PIGEA-14 ( polycystin-2 interactor, Golgi- and endoplasmic reticulum-associated protein with a molecular mass of 14 kDa). Molecular modeling only predicts a coiled-coil motif, but no other functional domains, in PIGEA-14. In a subsequent two-hybrid screen using PIGEA-14 as a bait, we found GM130, a component of the cis-compartment of the Golgi apparatus. Co-expression of the PIGEA-14 and PKD2 cDNAs in LLC-PK1 and HeLa cells resulted in a redistribution of PIGEA-14 and polycystin-2 to the trans-Golgi network, which suggests that PIGEA-14 plays an important role in regulating the intracellular location of polycystin-2 and possibly other intracellular proteins. Our results also indicate that the intracellular trafficking of polycystin-2 is regulated both at the level of the endoplasmic reticulum and that of the trans-Golgi network.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | KIDNEY-DISEASE PROTEIN; CATION CHANNEL; PKD2; GENE; IDENTIFICATION; INTERACTS; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie > Prof. Dr. Ralph Witzgall |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Jul 2021 08:52 |
| Last Modified: | 05 Jul 2021 08:52 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37344 |
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