Klouche, Mariam and Peri, G. and Knabbe, C. and Eckstein, H. H. and Schmid, Franz-Xaver and Schmitz, Gerd and Mantovani, A. (2004) Modified atherogenic lipoproteins induce expression of pentraxin-3 by human vascular smooth muscle cells. ATHEROSCLEROSIS, 175 (2). pp. 221-228. ISSN 0021-9150,
Full text not available from this repository. (Request a copy)Abstract
Inflammation is a critical contributing factor to the development and the progression of atherosclerosis. Recently, the acute-phase protein pentraxin-3 (PTX3), which has C-terminal sequence homology with the classic pentraxin C-reactive protein (CRP), was described to be increased in patients with myocardial infarction. In this study, we have investigated the capacity of human primary vascular smooth muscle cells (VSMC), derived from arterial specimens of ten different patients, to express PTX3 after incubation with atherogenic lipoproteins. Enzymatically degraded LDL (E-LDL), which is present in human early lesions, mediated a rapid cholesterol loading and foam cell transformation of primary VSMC, which was paralleled by a marked dose- and time-dependent expression of PTX3 mRNA and release of the acute-phase protein. Expression of PTX3 mRNA was delayed and remained almost undetectable for up to 6 h of incubation with E-LDL. However. during extended exposure to E-LDL for more than 24 h, PTX3 mRNA expression increased by more than 15-fold in VSMC foam cells, which was reflected by a concomitant release of up to 211 ng/ml PTX3 protein. We provide evidence for marked expression of PTX3 by VSMC induced by degraded lipoproteins, which may lead to an in situ vascular acute-phase reaction, contributing to the inflammatory pathogenesis of atherosclerosis. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LOW-DENSITY-LIPOPROTEIN; C-REACTIVE PROTEIN; HUMAN ENDOTHELIAL-CELLS; ACUTE-PHASE PROTEINS; AMYLOID-P COMPONENT; LONG PENTRAXIN; MYOCARDIAL-INFARCTION; APOPTOTIC CELLS; OXIDIZED LDL; PTX3; atherosclerosis; smooth muscle; E-LDL; acute-phase protein; PTX3 |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Herz-, Thorax- und herznahe Gefäßchirurgie Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Jul 2021 08:23 |
| Last Modified: | 05 Jul 2021 08:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37362 |
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