Krebs, Angelika and Durchschlag, Helmut and Zipper, Peter (2004) Small angle x-ray scattering studies and modeling of Eudistylia vancouverii chlorocruorin and Macrobdella decora hemoglobin. BIOPHYSICAL JOURNAL, 87 (2). pp. 1173-1185. ISSN 0006-3495,
Full text not available from this repository. (Request a copy)Abstract
Annelids possess giant extracellular oxygen carriers that exhibit a hexagonal bilayer appearance and have molecular masses of similar to3.5 MDa. By small angle x-ray scattering (SAXS), Eudistylia vancouverii chlorocruorin and Macrobdella decora hemoglobin were investigated in solution. On the basis of the experimental SAXS data, three-dimensional models were established in a two-step approach (trial and error and averaging). The main differences between the complexes concern the structure of their central part and the subunit architecture. Usage of our SAXS models as templates for automated model generation (program DAMMIN) led to refined models that fit perfectly the experimental data. Special attention was paid to the inhomogeneous density distribution observed within the complexes. DAMMIN models without a priori information could not reproducibly locate low-density areas. The usage of templates, however, improved the results considerably, in particular by applying electron microscopy-based templates. Biologically relevant information on the presence of low-density areas and hints for their presumable location could be drawn from SAXS and sophisticated modeling approaches. Provided that different models are analyzed carefully, this obviously opens a way to gain additional biologically relevant structural information from SAXS data.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LUMBRICUS-TERRESTRIS HEMOGLOBIN; ELECTRON-MICROSCOPIC STRUCTURES; GIANT EXTRACELLULAR HEMOGLOBIN; MASS-SPECTROMETRIC COMPOSITION; 3-DIMENSIONAL RECONSTRUCTION; QUATERNARY STRUCTURE; LINKER CHAINS; EARTHWORM ERYTHROCRUORIN; CRYOELECTRON MICROSCOPY; HYDRODYNAMIC PROPERTIES; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Jul 2021 09:33 |
| Last Modified: | 05 Jul 2021 09:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37364 |
Actions (login required)
 |
View Item |
