Hoffmann, Petra and Eder, Rüdiger and Kunz-Schughart, Leoni A. and Andreesen, Reinhard and Edinger, Matthias (2004) Large-scale in vitro expansion of polyclonal human CD4(+)CD25(high) regulatory T cells. BLOOD, 104 (3). pp. 895-903. ISSN 0006-4971, 1528-0020
Full text not available from this repository. (Request a copy)Abstract
CD4(+)CD25(+) regulatory T (T-reg) cells are pivotal for the maintenance of self-tolerance, and their adoptive transfer gives protection from autoimmune diseases and pathogenic alloresponses after solid organ or bone marrow transplantation in murine model systems. In vitro, human CD4(+)CD25(+) T-reg cells display phenotypic and functional characteristics similar to those of murine CD4(+)CD25(+) T-reg cells: namely, hyporesponsiveness to T-cell receptor (TCR) stimulation and suppression of CD25(-) T cells. Thus far, the detailed characterization and potential clinlcal application of human CD4(+)CD25(+) T-reg cells have been hampered by their paucity in peripheral blood and the lack of appropriate expansion protocols. Here we describe the up to 40 000-fold expansion of highly purified human CD4(+)CD25(high) T cells in vitro through the use of artificial antigen-presenting cells for repeated stimulation via CD3 and CD28 in the presence of high-dose interleukin 2 (IL-2). Expanded CD4(+)CD25(high) T cells were polyclonal, maintained their phenotype, exceeded the suppressive activity of freshly isolated CD4(+)CD25(high) T cells, and maintained expression of the lymph node homing receptors L-selectin (CD62L) and CCR7. The ability to rapidly expand human CD4(+)CD25(high) T-reg cells on a large scale will not only facilitate their further exploration but also accelerate their potential clinical application in T cell-mediated diseases and transplantation medicine.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | VERSUS-HOST-DISEASE; IMMUNOLOGICAL SELF-TOLERANCE; BONE-MARROW-TRANSPLANTATION; SECONDARY LYMPHOID ORGANS; EX-VIVO; TUMOR-IMMUNITY; AUTOIMMUNITY; INDUCTION; HOMEOSTASIS; MEMORY; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Jul 2021 09:39 |
| Last Modified: | 05 Jul 2021 09:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37366 |
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