Froehlich, Dieter and Wittmann, Sigrid and Rothe, Gregor and Sessler, Daniel I. and Vogel, Peter and Taeger, Kai (2004) Mild hyperthermia down-regulates receptor-dependent neutrophil function. ANESTHESIA AND ANALGESIA, 99 (1). pp. 284-292. ISSN 0003-2999,
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Mild hypothermia impairs resistance to infection and, reportedly, impairs phagocytosis and oxidative killing of unopsonized bacteria. We evaluated various functions at 33degrees-41degreesC in neutrophils taken from volunteers. Adhesion on endothelial cells was determined using light microscopy. Adhesion molecule expression and receptors, phagocytosis, and release of reactive oxidants were assessed using flow cytometric assays. Adhesion protein CD11b expression on resting neutrophils was temperature-independent. However, up-regulation of CD11b with tumor necrosis factor (TNF)-alpha was increased by hypothermia and decreased with hyperthermia. Neutrophil adhesion to either resting or activated endothelial cells was not temperature-dependent. Bacterial uptake was inversely related to temperature, more so with Escherichia coli than Staphylococcus aureus. Temperature dependence of phagocytosis occurred only with opsonized bacteria. Hypothermia slightly increased N-formyl-L-methionyl-L-leucyl-phenylalanine receptors on neutrophils: hyperthermia decreased expression, especially with TNF-alpha. N-formyl-L-methionyl-L-leucyl-phenylalanine-induced H2O2 production was inversely related to temperature, especially in the presence of TNF-alpha. Conversely, phorbol-13-myristate-12-acetate, an activator of protein kinase C, induced an extreme and homogenous release of reactive oxidants that increased with temperature. In contrast to nonreceptor-dependent phagocytosis and oxidative killing, several crucial receptor-dependent neutrophil activities show temperature-dependent regulation, with hypothermia increasing function. The temperature dependence of neutrophil function is thus more complicated than previously appreciated.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SURGICAL-WOUND INFECTION; PERIOPERATIVE HYPOTHERMIA; CYTOKINE PRODUCTION; ESCHERICHIA-COLI; OXYGEN-TENSION; PHAGOCYTES; EXPRESSION; REDUCE; CELLS; BURST; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Anästhesiologie Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Jul 2021 08:07 |
| Last Modified: | 07 Jul 2021 08:07 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37454 |
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