Fiehn, C. and Neumann, E. and Wunder, A. and Krienke, S. and Gay, S. and Mueller-Ladner, Ulf (2004) Methotrexate (MTX) and albumin coupled with MTX (MTX-HSA) suppress synovial fibroblast invasion and cartilage degradation in vivo. ANNALS OF THE RHEUMATIC DISEASES, 63 (7). pp. 884-886. ISSN 0003-4967, 1468-2060
Full text not available from this repository. (Request a copy)Abstract
Objective: To investigate the effect of methotrexate (MTX) and albumin coupled with methotrexate (MTX-HSA) on cartilage invasion and induction of perichondrocytic degradation by rheumatoid arthritis synovial fibroblasts ( RA SF) in vivo. Methods: Human cartilage and RA SF were co-transplanted in three groups of severe combined immunodeficient mice ( SCID), which received 1 mg/kg free MTX (n = 9), 1 mg/kg MTX-HSA ( n = 6), or 0.9% NaCl ( n = 5), respectively, intraperitoneally twice a week. After 4 weeks' treatment, the mice were killed and the implants analysed histologically. Results: The control group had a mean (SEM) score for cartilage invasion of RA SF of 2.0 (0.26) and for perichondrocytic cartilage degradation of 1.5 (0.34). In contrast, mice which received MTX showed a significantly reduced invasion (0.78 (0.14), p< 0.01) and a reduction in perichondrocytic cartilage degradation scores (0.69 (0.2), p< 0.05) in comparison with the control group. Mice treated with MTX-HSA also had significantly reduced scores for RA SF invasion into the cartilage (0.92 (0.41), p< 0.05) and for cartilage degradation (0.83 (0.44), p< 0.05) compared with controls. The effects of MTX and MTX-HSA were not significantly different between these two groups. Conclusion: Treatment with MTX or MTX-HSA significantly ameliorates cartilage destruction in the SCID mouse model for human RA.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RHEUMATOID-ARTHRITIS; CYTOKINE INHIBITORS; GENE-TRANSFER; BEARING RATS; INTERLEUKIN-1; DESTRUCTION; MODEL; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Jul 2021 08:10 |
| Last Modified: | 07 Jul 2021 08:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37465 |
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