Hierlmeier, Thomas and Reischl, Udo and Lang, Peter and Hebart, Holger and Stark, Maik and Kyme, Pierre and Autenrieth, Ingo B. (2004) Preliminary evaluation of one conventional nested and two real-time PCR assays for the detection of Toxoplasma gondii in immunocompromised patients. JOURNAL OF MEDICAL MICROBIOLOGY, 53 (7). pp. 629-632. ISSN 0022-2615,
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Toxoplasma reactivation is a serious complication in patients receiving allogenic stem cell transplantation. Real-time PCR assays allow a rapid diagnosis of toxoplasma. infection; however, no comparative data are available on the performance of real-time PCR protocols under routine conditions. Therefore, the aim of this study was to amplify Toxoplasma gondii DNA from routine samples of allogenic stem cell recipients using two real-time PCR assays on a LightCycler, and using conventional nested PCR. Conventional nested PCR revealed T. gondii DNA in 16 samples. Only 12 of the 16 samples yielded a positive result in both real-time PCRs. The accuracy of the conventional PCR results was demonstrated by direct sequencing. Amplification and detection of the amplicon was completed in only 1 h using the real-time PCR assays. Thus, real-time PCR substantially accelerates the detection of T. gondii DNA in the majority of positive specimens; however, conventional nested PCR is required for detection of T. gondii DNA in some samples.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TRANSFER HYBRIDIZATION PROBES; POLYMERASE CHAIN-REACTION; QUANTITATIVE PCR; DIAGNOSIS; DNA; TRANSPLANTATION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Jul 2021 07:22 |
| Last Modified: | 12 Jul 2021 07:22 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37501 |
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