Fath, Stephan and Kobor, Michael S. and Philippi, Anja and Greenblatt, Jack and Tschochner, Herbert (2004) Dephosphorylation of RNA polymerase I by Fcp1p is required for efficient rRNA synthesis. JOURNAL OF BIOLOGICAL CHEMISTRY, 279 (24). pp. 25251-25259. ISSN 0021-9258, 1083-351X
Full text not available from this repository. (Request a copy)Abstract
Differently phosphorylated forms of RNA polymerase (Pol) II are required to guide the enzyme through the transcription cycle. Here, we show that a phosphorylation/dephosphorylation cycle is also important for RNA polymerase I-dependent synthesis of rRNA precursors. A key component of the Pol II transcription system is Fcp1p, a phosphatase that dephosphorylates the C-terminal domain of the largest Pol II subunit. Fcp1p stimulates transcription elongation and is required for Pol II recycling after transcription termination. We found that Fcp1p is also part of the RNA Pol I transcription apparatus. Fcp1p is required for efficient rDNA transcription in vivo, and also, recombinant Fcp1p stimulates rRNA synthesis both in promoter-dependent and in nonspecific transcription assays in vitro. We demonstrate that Fcp1 activity is not involved in the formation of the initiation-active form of Pol I (the Pol I-Rrn3p complex) and propose that dephosphorylation of Pol I by Fcp1p facilitates chain elongation during rRNA synthesis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TATA-BINDING PROTEIN; UPSTREAM ACTIVATION FACTOR; TRANSCRIPTION FACTOR UBF; C-TERMINAL DOMAIN; SACCHAROMYCES-CEREVISIAE; RDNA TRANSCRIPTION; DEPENDENT REGULATION; TIF-IA; PREINITIATION COMPLEX; GENE-TRANSCRIPTION; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Herbert Tschochner |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Jul 2021 11:18 |
| Last Modified: | 13 Jul 2021 11:18 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37540 |
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