Nishino, Tsuyuki and Wedel, Thilo and Schmitt, Oliver and Buehlmeyer, Katja and Schoenfelder, Martin and Hirtreiter, Christian and Schulz, Thorsten and Kuehnel, Wolfgang and Michna, Horst (2004) Androgen-dependent morphology of prostates and seminal vesicles in the Hershberger Assay: Evaluation of immunohistochemical and morphometric parameters. ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER, 186 (3). pp. 247-253. ISSN 0940-9602,
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The aim of this study was to evaluate androgen-like effects using immunohistochemical and morphometric methods. Therefore, orchiectomized Wistar rats (n greater than or equal to 13) were treated s.c. with 1 mg/kg bw/day testosterone propionate (TP) for 7 days and compared to orchiectomized rats without TP substitution (OX) and to an untreated intact control group. Sections obtained from prostates and seminal vesicles were stained with polyclonal and monoclonal antibodies against the androgen receptor (AR) and assessed densitometrically (intensity of the immunoreaction) and morphometrically (epithelial height, luminal area). TP caused an enhancement of staining intensity and an increase in organ weights, epithelial height and luminal area. The use of proliferation markers (PCNA, MIB-5) showed also a highly significant increase of immunoreactive cells in TP-substituted orchiectomized rats compared with the OX group. Based on the present data, the densitometric analysis of AR-immunoreactivity as well as the assessment of proliferation markers, epithelial height and luminal area proved to be sensitive parameters for the evaluation of androgen effects on prostates and seminal vesicles. In further studies these parameters will be used to test several industrial xenooestrogens as well as phytooestrogens on their possible androgenic capacity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CELL NUCLEAR ANTIGEN; IMAGE-ANALYSIS; RAT-PROSTATE; PROLIFERATION; EXPRESSION; CARCINOMA; IMMUNOCYTOCHEMISTRY; CASTRATION; ANALYZER; SECTIONS; androgenic reactions; androgen receptor; prostate; seminal vesicle; densitometry; morphometry; proliferation markers |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Organische Chemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Jul 2021 12:28 |
| Last Modified: | 13 Jul 2021 12:28 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37552 |
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