Ryder, Edward and Blows, Fiona and Ashburner, Michael and Bautista-Llacer, Rosa and Coulson, Darin and Drummond, Jenny and Webster, Jane and Gubb, David and Gunton, Nicola and Johnson, Glynnis and O'Kane, Cahir J. and Huen, David and Sharma, Punita and Asztalos, Zoltan and Baisch, Heiko and Schulze, Janet and Kube, Maria and Kittlaus, Kathrin and Reuter, Gunter and Maroy, Peter and Szidonya, Janos and Rasmuson-Lestander, Asa and Ekstroem, Karin and Dickson, Barry and Hugentobler, Christoph and Stocker, Hugo and Hafen, Ernst and Lepesant, Jean Antoine and Pflugfelder, Gert and Heisenberg, Martin and Mechler, Bernard and Serras, Florenci and Corominas, Montserrat and Schneuwly, Stephan and Preat, Thomas and Roote, John and Russell, Steven (2004) The DrosDel collection: A set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster. GENETICS, 167 (2). pp. 797-813. ISSN 0016-6731, 1943-2631
Full text not available from this repository. (Request a copy)Abstract
We describe a collection of P-element insertions that have considerable utility for generating custom chromosomal aberrations in Drosophila melanogaster. We have mobilized a pair of engineered P elements, p{RS3} and p{RS5}, to collect 3243 lines unambiguously mapped to the Drosophila genome sequence. The collection contains, on average, an element every 35 kb. We demonstrate the utility of the collection for generating custom chromosomal deletions that have their end points mapped, with base-pair resolution, to the genome sequence. The collection was generated in an isogenic strain, thus affording a uniform background for screens where sensitivity to genetic background is high. The entire collection, along with a computational and genetic toolbox for designing and generating custom deletions, is publicly available. Using the collection it is theoretically possible to generate >12,000 deletions between 1 bp and 1 Mb in size by simple eye color selection. In addition, a further 37,000 deletions, selectable by molecular screening, may be generated. We are now rising the collection to generate a second-generation deficiency kit that is precisely mapped to the genome sequence.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GENE ACTIVITY; GENOME; REQUIREMENTS; SEQUENCE; DESIGN; REGION; DNA; |
| Subjects: | 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie > Entwicklungsbiologie (Prof. Dr. Stephan Schneuwly) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Jul 2021 15:09 |
| Last Modified: | 14 Jul 2021 15:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37577 |
Actions (login required)
 |
View Item |
