Schaeffler, Andreas and Ehling, A. and Neumann, E. and Herfarth, H. and Paul, G. and Tarner, I. and Gay, S. and Buechler, C. and Schoelmerich, Juergen and Mueller-Ladner, Ulf (2004) Role of specificity protein-1, PPAR gamma, and pituitary protein transcription factor-1 in transcriptional regulation of the murine CORS-26 promoter. BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1678 (2-3). pp. 150-156. ISSN 0167-4781
Full text not available from this repository. (Request a copy)Abstract
The collagenous repeat-containing sequence of 26-kDa protein (CORS-26) was recently described as a new gene that is induced during adipocyte differentiation. Since the transcription factors specificity protein-1 (SP-1) and PPARgamma have been demonstrated to modulate transcriptional activation of adipocytic genes, we investigated the putative role of SPA and PPARgamma in the regulation of the murine CORS-26 promoter. Computer-based sequence analysis revealed two putative SP-1 binding sites and binding sites for PPARgamma and Pit-1 within the TATA-box containing promoter. Electrophoretic mobility shift assays (EMSA) with nuclear extracts from 3T3-L1 adipocytes and appropriate promoter fragments demonstrated that SP-1 binds specifically to both SP-1 binding sites. Specificity was demonstrated by (i) the appearance of supershift bands, (ii) competition experiments and, (iii) by using oligonucleotides carrying mutated SP-1 binding sites. Functional promoter activity was analyzed by Luciferase reporter gene assays and SP-1 was shown to exert inhibitory effects on the transcriptional activation of the murine CORS-26 gene. Additionally, specific binding activity of PPARgamma and Pit-1 to the CORS-26 promoter was demonstrated. Taken together, the present data demonstrate the functionality of the proximal murine CORS-26 promoter, which is regulated specifically by two SPA binding sites via SP-3-independent repressive effects of SP-1 on transcriptional activation. Pit-1 and PPARgamma can bind specifically to the promoter and might play an additive functional role in gene regulation of murine CORS-26. (C) 2004 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DNA-BINDING SITES; ADIPOSE CELL LINE; FACTOR SP1; GENE; ACTIVATION; DIFFERENTIATION; EXPRESSION; DOMAINS; FAMILY; REPRESSION; CORS-26; promoter; SP-1; Pit-1; PPAR-gamma; cartilage; adipocyte |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Jul 2021 06:14 |
| Last Modified: | 20 Jul 2021 06:14 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37635 |
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