Schreiber, R. and Boucherot, A. and Muerle, B. and Sun, J. and Kunzelmann, Karl (2004) Control of epithelial ion transport by Cl- and PDZ proteins. JOURNAL OF MEMBRANE BIOLOGY, 199 (2). pp. 85-98. ISSN 0022-2631, 1432-1424
Full text not available from this repository. (Request a copy)Abstract
Inhibition of epithelial Na+ channels (ENaC) by the cystic fibrosis transmembrane conductance regulator (CFTR) has been demonstrated previously. Recent studies suggested a role of cytosolic Cl- for the interaction of CFTR with ENaC, when studied in Xenopus oocytes. In the present study we demonstrate that the Na+/H+-exchanger regulator factor (NHERF) controls expression of CFTR in mouse collecting duct cells. Inhibition of NHERF largely attenuates CFTR expression, which is paralleled by enhanced Ca2+-dependent Cl- secretion and augmented Na+ absorption by the ENaC. It is further demonstrated that epithelial Na+ absorption and ENaC are inhibited by cytosolic Cl- and that stimulation by secretagogues enhances the intracellular Cl- concentration. Thus, the data provide a clue to the question, how epithelial cells can operate as both absorptive and secretory units: Increase in intracellula Cl- Cl- during activation of secretion will inhibit ENaC and switch epithelial transport from salt absorption to Cl- secretion.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TRANSMEMBRANE CONDUCTANCE REGULATOR; COLLECTING DUCT CELLS; CFTR CHLORIDE CHANNEL; CYSTIC-FIBROSIS; NA+ CHANNELS; KINASE-A; XENOPUS OOCYTES; PURINERGIC STIMULATION; INTERACTING DOMAIN; SODIUM-CHANNELS; CFTR; ENaC; intracellular chloride; PDZ domain; NHERF; epithelial transport |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Karl Kunzelmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Jul 2021 06:39 |
| Last Modified: | 20 Jul 2021 06:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37640 |
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