Sandner, Peter and Hofbauer, Karl-Heinz and Tinel, Hanna and Kurtz, Armin and Thiesson, Helle C. and Ottosen, Peter D. and Walter, Steen and Skott, Ole and Jensen, Boye L. (2004) Expression of adrenomedullin in hypoxic and ischemic rat kidneys and human kidneys with arterial stenosis. AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 286 (5). R942-R951. ISSN 0363-6119, 1522-1490
Full text not available from this repository. (Request a copy)Abstract
To investigate regional aspects of hypoxic regulation of adrenomedullin (AM) in kidneys, we mapped the distribution of AM in the rat kidney after hypoxia (normobaric hypoxic hypoxia, carbon monoxide, and CoCl2 for 6 h), anemia (hematocrit lowered by bleeding) and after global transient ischemia for 1 h (unilateral renal artery occlusion and reperfusion for 6 and 24 h) and segmental infarct (6 and 24 h). AM expression and localization was determined in normal human kidneys and in kidneys with arterial stenosis. Hypoxia stimulated AM mRNA expression significantly in rat inner medulla (CO 13 times, 8% O-2 6 times, and CoCl2 8 times), followed by the outer medulla and cortex. AM mRNA level was significantly elevated in response to anemia and occlusion-reperfusion. Immunoreactive AM was associated with the thin limbs of Henle's loop, distal convoluted tubule, collecting ducts, papilla surface epithelium, and urothelium. AM labeling was prominent in the inner medulla after CO and in the outer medulla after occlusion-reperfusion. The infarct border zone was strongly labeled for AM. In cultured inner medullary collecting duct cells, AM mRNA was significantly increased by hypoxia. AM mRNA was equally distributed in human kidney and AM was localized as in the rat kidney. In human kidneys with artery stenosis, AM mRNA was not significantly enhanced compared with controls, but AM immunoreactivity was observed in tubules, vessels, and glomerular cells. In summary, AM expression was increased in the rat kidney in response to hypoxic and ischemic hypoxia in keeping with oxygen gradients. AM was widely distributed in the human kidney with arterial stenosis. AM may play a significant role to counteract hypoxia in the kidney.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HYPERTENSIVE RATS; INDUCIBLE FACTOR-1-ALPHA; HYPOTENSIVE PEPTIDE; COLLECTING DUCT; RENAL INJURY; CELLS; PLASMA; TISSUE; GENE; HEMODYNAMICS; anemia; hypertension; infarct; oxygen |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Jul 2021 08:28 |
| Last Modified: | 20 Jul 2021 08:28 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37653 |
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