Bucher, Michael (2004) Expression of vasoconstrictive receptors during experimental endotoxoemia. ANASTHESIOLOGIE & INTENSIVMEDIZIN, 45 (5). 247-+. ISSN 0170-5334
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The diminished vasoconstrictive activity of norepinephrine, angiotensin II or vasopressin directed our interest towards the regulation of the receptors for vasoconstrictors during the conditions of experimental endotoxaemia in vivo and in vitro. Male Sprague-Dawley rats were injected with lipopolysaccharide (10 mg/kg) i.v. to induce endotoxaemia and systolic blood pressure was measured each hour. The animals were sacrificed after 6, 12 or 24 hours and organs were removed. Gene expression of vasoconstrictive receptors such as angiotensin II type 1-(AT(1))-receptors, vasopressin V-1A-receptors and alpha(1)-adrenergic receptors were determined with specific RNase-protection-assay. Tissue-concentrations of the proinflammatory cytokines IL-1beta and TNF-alpha and of catecholamines were measured using ELISA or HPLC, respectively. Plasma-renin-activity, plasma-angiotensin II- und plasma-aldosteron-concentrations were determined with RIA. In addition, blood-pressure-dose-response studies with angiotensin II and a selective V-1-receptor-agonist were carried out after induction of experimental endotoxaemia compared to vehicle-injected rats. Rat renal mesangial cells and aortal vascular smooth muscle cells (cell line A7r5) were incubated with proinflammatory cytokines and NO-donators and gene and protein expression of vasoconstrictive receptors were determined using RNase-protection-assay and radioligand-binding studies, respectively.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NITRIC-OXIDE SYNTHASE; SMOOTH-MUSCLE CELLS; SEVERE SEPTIC SHOCK; SYSTEMIC INFLAMMATORY RESPONSE; ANGIOTENSIN-II RECEPTORS; DOWN-REGULATION; RAT MODEL; ALPHA-1-ADRENERGIC RECEPTORS; ARGININE-VASOPRESSIN; SELECTIVE INHIBITOR; cytokines; nitric oxide; blood pressure; catecholamines; vasopressin; angiotensin |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Anästhesiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Jul 2021 08:39 |
| Last Modified: | 20 Jul 2021 08:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37655 |
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