Schulz, Christian and Kraetzel, Kirsten and Wolf, Konrad and Schroll, Stephan and Koehler, Martina and Pfeifer, Michael (2004) Activation of bronchial epithelial cells in smokers without airway obstruction and patients with COPD. CHEST, 125 (5). pp. 1706-1713. ISSN 0012-3692
Full text not available from this repository. (Request a copy)Abstract
Study objective: The aim of this study was to investigate the basal level as well as the tumor necrosis factor (TNF)-alpha- and interferon (IFN)-gamma-induced expression and release of the neutrophil chemoattractants interleukin (IL)-8 and growth-related oncogene (GRO)-alpha in primary bronchial epithelial cells (PBECs) from smokers without airflow obstruction and patients with COPD. In addition, the expression of both TNF-alpha-receptor subtypes-p55 TNF-receptor subtype (TNF-R55) and p75 TNF-receptor subtype (TNF-R75)-was quantified in PBECs. Design: PBECs from eight smokers without airflow limitation and eight patients with COPD were stimulated with 50 ng/mL of TNF and 200 U/mL of IFN-gamma for 4 h along with unstimulated time controls. The transcriptional expression and protein release were quantitatively assessed by means of real-time polymerase chain reaction and enzyme-linked immunosorbent assay. Results: Basal level messenger RNA (mRNA) expression and protein release of IL-8 and GRO-alpha were not significantly different between both groups, although a trend toward higher IL-8 levels was seen in patients with COPD. TNF-alpha induced significantly higher mRNA amounts of IL-8 (p = 0.005) and GRO-alpha (p = 0.007) in patients with COPD. This was accompanied by higher protein release data for IL-8 (p = 0.005) and GRO-alpha (p = 0.007). IFN-gamma had no significant effect on the mRNA expression and protein release of IL-8 and GRO-a in either group. TNF-R55 and TNF-R75 were detectable in PBECs. However, no significant differences were found between both groups with respect to steady-state mRNA levels of TNF-alpha-receptor subtypes. Conclusion: PBECs from patients with COPD show significantly higher TNF-alpha-induced release of the neutrophil chemoattractant CXC-chemokines IL-8 and GRO-alpha compared to smokers without airflow limitation. This increased activation of PBECs may contribute to the predominance of neutrophils seen in the airway lumen of patients with COPD.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PULMONARY-DISEASE; INTERFERON-GAMMA; INTERLEUKIN-8; INFLAMMATION; RELEASE; SPUTUM; ASTHMA; ALPHA; NEUTROPHILS; CHEMOKINES; bronchial epithelium; COPD; growth-related oncogene-alpha; interleukin-8; smoking |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Jul 2021 09:22 |
| Last Modified: | 20 Jul 2021 09:22 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37668 |
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