Gess, Bernhard and Hofbauer, Karl-Heinz and Deutzmann, R. and Kurtz, Armin (2004) Hypoxia up-regulates triosephosphate isomerase expression via a HIF-dependent pathway. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 448 (2). pp. 175-180. ISSN 0031-6768, 1432-2013
Full text not available from this repository. (Request a copy)Abstract
The glycolytic enzyme triosephosphate isomerase (TPI) catalyses the reversible conversion of dihydroxyacetone phosphate into glyceraldehyde-3-phosphate. We report here that the expression of TPI at both the mRNA and protein levels is increased by hypoxia in vivo and in vitro. The temporal pattern of hypoxic TPI induction is very similar to that of genes triggered by the hypoxia-inducible transcription factor (HIF) and is mimicked characteristically by cobalt and by deferoxamine, but is absent in cells with a defective aryl hydrocarbon receptor nuclear translocator (ARNT, here HIF-1beta) and in cells lacking HIF-1alpha protein. We conclude from these findings that the expression of TPI is regulated via the HIF pathway and thus belongs to the family of classic oxygen-regulated genes. The physiological meaning of an increased expression of TPI in hypoxygenated tissues is probably to increase the flow of triosephosphates through the glycolytic cascade thus leading to an increase of anaerobic energy generation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INDUCIBLE FACTOR-I; MOUSE HEPATOMA-CELLS; GLYCOLYTIC-ENZYMES; O-2 HOMEOSTASIS; GENE-EXPRESSION; FACTOR 1-ALPHA; ERYTHROPOIETIN; HIF-1-ALPHA; SIMILARITIES; MODULATION; glycolysis; deferoxamine; cobalt; oxygen tension |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Rainer Deutzmann Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Jul 2021 06:01 |
| Last Modified: | 21 Jul 2021 06:01 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37719 |
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