Bier, Mirko and Fath, Stephan and Tschochner, Herbert (2004) The composition of the RNA polymerase I transcription machinery switches from initiation to elongation mode. FEBS LETTERS, 564 (1-2). pp. 41-46. ISSN 1873-3468
Full text not available from this repository. (Request a copy)Abstract
The amounts of RNA polymerase I (Pol I) and basal rDNA transcription factors were determined in yeast whole cell extracts. A 17-fold excess of Pol I was found compared to the Pol I-specific initiation factors upstream activating factor (UAF) and core factor (CF) which underlines that both initiation factors interact with a minor fraction of Pol I when rDNA transcription is active. Surprisingly, Rrn3p, another Pol I-specific initiation factor, is more abundant in cell lysates than UAF and CF. Our analyses revealed that a large fraction of cellular Rrn3p is not associated with Pol I. However, the amount of initiation-active Rrn3p which forms a stable complex with Pol I corresponds to the levels of UAF and CF which have been shown to bind the promoter. Initiation-active Rrn3p dissociates from the template during or immediately after Pol I has switched from initiation to elongation. Our data support a model in which the elongating Pol I leaves the initiation factors UAF, CF and Rrn3p close by the promoter. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SACCHAROMYCES-CEREVISIAE; YEAST; RECRUITMENT; PROTEIN; RRN3; GENE; RDNA; EXPRESSION; INTERACTS; TEMPLATE; ribosome synthesis; nucleolus; rDNA; in vitro transcription; promoter; RNA polymerase I; Rrn3p; Saccharomyces cerevisiae |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Herbert Tschochner |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Jul 2021 08:09 |
| Last Modified: | 21 Jul 2021 08:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37735 |
Actions (login required)
 |
View Item |
