Hausmann, Martin and Obermeier, F. and Schreiter, K. and Spottl, T. and Falk, W. and Schoelmerich, Juergen and Herfarth, H. and Saftig, P. and Rogler, G. (2004) Cathepsin D is up-regulated in inflammatory bowel disease macrophages. CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 136 (1). pp. 157-167. ISSN 0009-9104, 1365-2249
Full text not available from this repository. (Request a copy)Abstract
Down-regulation of receptors involved in the recognition or transmission of inflammatory signals and a reduced responsiveness support the concept that macrophages are 'desensitized' during their differentiation in the intestinal mucosa. During inflammatory bowel disease (IBD) intestinal macrophages (IMACs) change to a reactive or 'aggressive' type. After having established a method of isolation and purification of IMACs, message for cathepsin D was one of the mRNAs we found to be up-regulated in a subtractive hybridization of Crohn's disease (CD) macrophages versus IMACs from control mucosa. The expression of cathepsin D in intestinal mucosa was analysed by immunohistochemistry in biopsies from IBD and control patients and in a mouse model of dextran sulphate sodium (DSS)-induced acute and chronic colitis. IMACs were isolated and purified from normal and inflamed mucosa by immunomagnetic beads armed with a CD33 antibody. RT-PCR was performed for cathepsin D mRNA. Results were confirmed by Northern blot and flow cytometrical analysis. Immunohistochemistry revealed a significant increase in the cathepsin D protein expression in inflamed intestinal mucosa from IBD patients compared to non-inflamed mucosa. No cathepsin D polymerase chain reaction (PCR) product could be obtained with mRNA from CD33-positive IMACs from normal mucosa. Reverse transcription (RT)-PCR showed an induction of mRNA for cathepsin D in purified IMACs from IBD patients. Northern blot and flow cytometry analysis confirmed these results. Cathepsin D protein was also found in intestinal mucosa in acute and chronic DSS-colitis but was absent in normal mucosa. This study shows that expression of cathepsin D is induced in inflammation-associated IMACs. The presence of cathepsin D might contribute to the mucosal damage in IBD.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ANTIGEN PRESENTATION; BREAST-CANCER; EXTRACELLULAR-MATRIX; COSTIMULATORY MOLECULES; INTESTINAL MACROPHAGES; BONE-RESORPTION; CELLS; EXPRESSION; PROTEASES; MUCOSA; cathepsin D; inflammatory bowel disease; macrophages |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Jul 2021 05:04 |
| Last Modified: | 26 Jul 2021 05:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37771 |
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