Wellinghausen, Nele and Wirths, Beate and Franz, Axel R. and Karolyi, Lothar and Marre, Reinhard and Reischl, Udo (2004) Algorithm for the identification of bacterial pathogens in positive blood cultures by real-time LightCycler polymerase chain reaction (PCR) with sequence-specific probes. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 48 (4). pp. 229-241. ISSN 0732-8893
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We developed real-time polymerase chain reaction (PCR) assays for rapid detection of the most common and clinically relevant bacteria in positive blood Culture bottles. including Staphylococcus spp., S. epidermidis, S. aureus, Enterococcus spp. (including differentiation of E. faecalis and E. faecium). Streptococcus spp., Streptococcus agalactiae, Enterobacteriaceae, E. coli, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Acinetobacter spp., Bacteroides spp., Haemophilus influenzae, and Neisseria meningitidis. A total of 507 positive blood cultures were investigated according to a specific PCR algorithm based on the microscopic result of the blood culture, and the PCR results were compared to the Culture results. Apart from-cross reactions between E. coli and Chryseomonas luteola and Enterococcus faecium and E. durans. the PCR assay correctly identified all bacteria in the blood Cultures and did not show any false-positive results. Regarding blood cultures positive with a single species of bacteria (n = 474), 98.3% of all bacteria were correctly detected by the PCR algorithm within a few hours. However. in mixed infections, the sensitivity was lower. The PCR algorithm is well suited for rapid identification of the most common bacteria in positive blood Cultures. (C) 2004 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RIBOSOMAL-RNA GENE; RAPID IDENTIFICATION; SEPTIC SHOCK; SEPSIS; DNA; HYBRIDIZATION; DIAGNOSIS; 16S rRNA gene; blood culture; LightCycler (TM) PCR; TaqMan PCR |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Jul 2021 05:12 |
| Last Modified: | 26 Jul 2021 05:12 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37774 |
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