Stolz, Jürgen and Caspari, Thomas and Carr, Antony M. and Sauer, Norbert (2004) Cell division defects of Schizosaccharomyces pombe liz1(-) mutants are caused by defects in pantothenate uptake. EUKARYOTIC CELL, 3 (2). pp. 406-412. ISSN 1535-9778
Full text not available from this repository. (Request a copy)Abstract
The liz1(+) gene of the fission yeast Schizosaccharomyces pombe was previously identified by complementation of a mutation that causes abnormal mitosis when ribonucleotide reductase is inhibited. Liz1 has similarity to transport proteins from Saccharomyces cerevisiae, but the potential substrate and its connection to the cell division cycle remain elusive. We report here that liz1+ encodes a plasma membrane-localized active transport protein for the vitamin pantothenate, the precursor of coenzyme A (CoA). Liz1 is required for pantothenate uptake at low extracellular concentrations. A lack of pantothenate uptake results in three phenotypes: (i) slow growth, (ii) delayed septation, and (iii) aberrant mitosis in the presence of hydroxyurea (HU). All three phenotypes are suppressed by high extracellular concentrations of pantothenate, where pantothenate uptake occurs by passive diffusion. liz1Delta mutants are viable because they can synthesize pantothenate from uracil as an endogenous source. The use of uracil for both pantothenate biosynthesis and deoxyribonucleotide generation provides an explanation for the aberrant mitosis in the presence of HU. HU blocks ribonucleotide reductase, and we propose that the accumulation of ribonucleotides reduces uracil biosynthesis by feedback inhibition of aspartate transcarbamoylase. Thus, the addition of HU to liz1Delta mutants results in a shortage of pantothenate. Because liz1Delta mutants show striking similarities to mutants with defects in fatty acid biosynthesis, we propose that the shortage of pantothenate compromises fatty acid synthesis, resulting in slow growth and mitotic defects.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DNA-DAMAGE CHECKPOINTS; ACETYL-COA CARBOXYLASE; FATTY-ACID SYNTHESIS; SACCHAROMYCES-CEREVISIAE; FISSION YEAST; ASPARTATE TRANSCARBAMOYLASE; ESCHERICHIA-COLI; S-PHASE; GENE; IDENTIFICATION; |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Jul 2021 05:15 |
| Last Modified: | 26 Jul 2021 05:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37775 |
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