Mander, Gerd J. and Pierik, Antonio J. and Huber, Harald and Hedderich, Reinhard (2004) Two distinct heterodisulfide reductase-like enzymes in the sulfate-reducing archaeon Archaeoglobus profundus. EUROPEAN JOURNAL OF BIOCHEMISTRY, 271 (6). pp. 1106-1116. ISSN 0014-2956
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Heterodisulfide reductase (Hdr) is a unique disulfide reductase that plays a key role in the energy metabolism of methanogenic archaea. Two types of Hdr have been identified and characterized from distantly related methanogens. Here we show that the sulfate-reducing archaeon Archaeoglobus profundus cultivated on H-2/sulfate forms enzymes related to both types of Hdr. From the membrane fraction of A. profundus, a two-subunit enzyme (HmeCD) composed of a b-type cytochrome and a hydrophilic iron-sulfur protein was isolated. The amino-terminal sequences of these subunits revealed high sequence identities to subunits HmeC and HmeD of the Hme complex from A. fulgidus. HmeC and HmeD in turn are closely related to subunits HdrE and HdrD of Hdr from Methanosarcina spp. From the soluble fraction of A. profundus a six-subunit enzyme complex (Mvh:Hdl) containing Ni, iron-sulfur clusters and FAD was isolated. Via amino-terminal sequencing, the encoding genes were identified in the genome of the closely related species A. fulgidus in which these genes are clustered. They encode a three-subunit [NiFe] hydrogenase with high sequence identity to the F-420-nonreducing hydrogenase from Methanothermobacter spp. while the remaining three polypeptides are related to the three-subunit heterodisulfide reductase from Methanothermobacter spp. The oxidized enzyme exhibited an unusual EPR spectrum with g(xyz) = 2.014, 1.939 and 1.895 similar to that observed for oxidized Hme and Hdr. Upon reduction with H-2 this signal was no longer detectable.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COMPLETE GENOME SEQUENCE; DESULFOVIBRIO-VULGARIS HILDENBOROUGH; MEMBRANE-BOUND HYDROGENASE; IRON-SULFUR CLUSTERS; METHANOBACTERIUM-THERMOAUTOTROPHICUM; METHANOSARCINA-MAZEI; CYTOCHROME-B; ACTIVE-SITE; METHANOTHERMOBACTER-MARBURGENSIS; METHANOGENIC ARCHAEA; Archaeoglobus; heterodisulfide reductase; Hmc complex; iron-sulfur proteins; sulfate-reducing bacteria |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Jul 2021 08:32 |
| Last Modified: | 27 Jul 2021 08:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37913 |
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