Torner, Luz and Maloumby, Rodriguez and Nava, Gabriel and Aranda, Jorge and Clapp, Carmen and Neumann, Inga D. (2004) In vivo release and gene upregulation of brain prolactin in response to physiological stimuli. EUROPEAN JOURNAL OF NEUROSCIENCE, 19 (6). pp. 1601-1608. ISSN 0953-816X
Full text not available from this repository. (Request a copy)Abstract
Although prolactin (PRL) actions and expression in the brain have been shown, dynamic changes in its intracerebral release and gene expression have still not been demonstrated. Using push-pull perfusion, the in vivo release of PRL was monitored within the paraventricular nucleus (PVN) and medial preoptic area (MPOA) of virgin female, lactating and male rats in response to various stimuli. Perfusion with a depolarizing medium (56 mm K+) increased local release of PRL within both the PVN (P< 0.05) and MPOA (P< 0.05) of urethane-anaesthetized rats, indicating release from excitable neuronal structures. The PRL in perfusates was verified by radioimmunoassay, Nb2 cell bioassays and western blot. Systemic osmotic stimulation (3 M NaCl i.p., 8 mL/kg b.w.) raised PRL concentration in plasma (P< 0.01) but not within the PVN, suggesting independent release from the pituitary and in distinct brain regions. Immobilization for 30 min increased PRL release within the PVN (P< 0.05) and the MPOA (P< 0.01) of virgin female and male (P < 0.05 each) rats and increased hypothalamic PRL mRNA expression (P= 0.008) after 30 and 90 min as revealed by real-time polymerase chain reaction. This indicates a stress-induced activation of both PRL release from and synthesis in hypothalamic neurons. Additionally, PRL was significantly released within, but not outside, the PVN (P< 0.01) and the MPOA (P< 0.05) of lactating rats during suckling and this was accompanied by a significant increase of PRL mRNA (P < 0.05) in the hypothalamus 60 min after suckling. This is the first demonstration of stimulus-induced, locally restricted release and gene upregulation of PRL within the brain, emphasizing the involvement of this 'novel' neuropeptide in various brain functions.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HYPOTHALAMIC PARAVENTRICULAR NUCLEUS; OXYTOCIN MESSENGER-RNA; PUSH-PULL PERFUSION; MATERNAL-BEHAVIOR; FEMALE RATS; IMMUNOREACTIVE PROLACTIN; CEREBROSPINAL-FLUID; C-FOS; MAGNOCELLULAR NEURONS; PERIPHERAL RELEASE; hypothalamus; medial preoptic area; neuropeptide; paraventricular; push-pull; rat; stress; suckling |
| Subjects: | 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie > Tierphysiologie/Neurobiologie (Prof. Dr. Inga Neumann) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Jul 2021 09:08 |
| Last Modified: | 27 Jul 2021 09:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37917 |
Actions (login required)
 |
View Item |
