Rothhammer, T. and Hahne, J. C. and Florin, A. and Poser, I. and Soncin, F. and Wernert, N. and Bosserhoff, Anja-Katrin (2004) The Ets-1 transcription factor is involved in the development and invasion of malignant melanoma. CELLULAR AND MOLECULAR LIFE SCIENCES, 61 (1). pp. 118-128. ISSN 1420-682X
Full text not available from this repository.Abstract
The Ets-1 transcription factor plays a role in tumor vascularization and invasion by regulating expression of matrix-degrading proteases in endothelial cells and fibroblasts in the tumor stroma. During early embryogenesis, Ets-1 is expressed in migrating neural crest cells from which melanocytes arise. In the present study, we analyzed Ets-1 expression in various melanocytic lesions and investigated its functional importance in malignant melanomas. We found that Ets-1 was upregulated both in vivo and in vitro in malignant melanoma, compared to benign melanocytic lesions and to primary melanocytes. Assessment of DNA-binding and transactivation assays documented a strong Ets activity in melanoma cells. Using an antisense strategy, the expression and activity of Ets-1 were reduced in the melanoma cell line Mel Im. This correlated with a diminished expression of several Ets-1 target genes known to be involved in invasion, such as MMP1, MMP3, uPA and integrin beta3. In line with these findings, the invasive potential of the melanoma cells measured in a Boyden Chamber model was reduced up to 60% after Ets-1 blockade. This can be attributed to the role of Ets-1 in transcriptional regulation of factors involved in invasion of melanoma cells. We conclude that over-expression of Ets-1 during melanoma development contributes to the malignant phenotype.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ENDOTHELIAL-CELLS; PLASMINOGEN-ACTIVATOR; MOLECULAR-BIOLOGY; EPITHELIAL-CELLS; TUMOR INVASION; MESSENGER-RNA; RETINOIC ACID; BREAST-CANCER; GENE FAMILY; EXPRESSION; malignant melanoma; Ets-1; proliferation; migration; invasion |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Aug 2021 08:09 |
| Last Modified: | 04 Aug 2021 08:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38127 |
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