Lommel, Mark and Bagnat, Michel and Strahl, Sabine (2004) Aberrant processing of the WSC family and Mid2p cell surface sensors results in cell death of Saccharomyces cerevisiae O-mannosylation mutants. MOLECULAR AND CELLULAR BIOLOGY, 24 (1). pp. 46-57. ISSN 0270-7306, 1098-5549
Full text not available from this repository. (Request a copy)Abstract
Protein O mannosylation is a crucial protein modification in uni- and multicellular eukaryotes. In humans, a lack of O-mannosyl glycans causes congenital muscular dystrophies that are associated with brain abnormalities. In yeast, protein O mannosylation is vital; however, it is not known why impaired 0 mannosylation results in cell death. To address this question, we analyzed the conditionally lethal Saccharomyces cerevisiae protein O-mannosyltransferase pmt2 pmt4Delta mutant. We found that pmt2 pmt4Delta cells lyse as small-budded cells in the absence of osmotic stabilization and that treatment with mating pheromone causes pheromone-induced cell death. These phenotypes are partially suppressed by overexpression of upstream elements of the protein kinase C (PKC1) cell integrity pathway, suggesting that the PKC1 pathway is defective in pmt2 pmt4Delta mutants. Congruently, induction of Mpk1p/Slt2p tyrosine phosphorylation does not occur in pmt2 pmt4Delta mutants during exposure to mating pheromone or elevated temperature. Detailed analyses of the plasma membrane sensors of the PKCI pathway revealed that Wsc1p, Wsc2p, and Mid2p are aberrantly processed in pmt mutants. Our data suggest that in yeast, Omannosylation increases the activity of Wsc1p, Wsc2p, and Mid2p by enhancing their stability. Reduced Omannosylation leads to incorrect proteolytic processing of these proteins, which in turn results in impaired activation of the PKCI pathway and finally causes cell death in the absence of osmotic stabilization.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN-KINASE-C; GENOME-WIDE ANALYSIS; MAP KINASE; WALL INTEGRITY; SIGNAL-TRANSDUCTION; MATING PHEROMONE; STRESS-RESPONSE; SHUTTLE VECTORS; GENE-EXPRESSION; EXCHANGE FACTOR; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Aug 2021 10:39 |
| Last Modified: | 04 Aug 2021 10:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38218 |
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