Silberhorn, Elisabeth and Schwartz, Uwe and Loeffler, Patrick and Schmitz, Samuel and Symelka, Anne and de Koning-Ward, Tania and Merkl, Rainer and Laengst, Gernot (2016) Plasmodium falciparum Nucleosomes Exhibit Reduced Stability and Lost Sequence Dependent Nucleosome Positioning. PLOS PATHOGENS, 12 (12): e1006080. ISSN 1553-7366, 1553-7374
Full text not available from this repository. (Request a copy)Abstract
The packaging and organization of genomic DNA into chromatin represents an additional regulatory layer of gene expression, with specific nucleosome positions that restrict the accessibility of regulatory DNA elements. The mechanisms that position nucleosomes in vivo are thought to depend on the biophysical properties of the histones, sequence patterns, like phased di-nucleotide repeats and the architecture of the histone octamer that folds DNA in 1.65 tight turns. Comparative studies of human and P. falciparum histones reveal that the latter have a strongly reduced ability to recognize internal sequence dependent nucleosome positioning signals. In contrast, the nucleosomes are positioned by AT-repeat sequences flanking nucleosomes in vivo and in vitro. Further, the strong sequence variations in the plasmodium histones, compared to other mammalian histones, do not present adaptations to its AT-rich genome. Human and parasite histones bind with higher affinity to GC-rich DNA and with lower affinity to AT-rich DNA. However, the plasmodium nucleosomes are overall less stable, with increased temperature induced mobility, decreased salt stability of the histones H2A and H2B and considerable reduced binding affinity to GC-rich DNA, as compared with the human nucleosomes. In addition, we show that plasmodium histone octamers form the shortest known nucleosome repeat length (155bp) in vitro and in vivo. Our data suggest that the biochemical properties of the parasite histones are distinct from the typical characteristics of other eukaryotic histones and these properties reflect the increased accessibility of the P. falciparum genome.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN MALARIA PARASITE; CHICKEN ERYTHROCYTE CHROMATIN; HISTONE OCTAMER; CORE PARTICLES; REGULATORY SEQUENCES; IN-VITRO; DNA; TRANSCRIPTION; EXPRESSION; OCCUPANCY; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Gernot Längst |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 Apr 2019 08:47 |
| Last Modified: | 11 Apr 2019 08:47 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3834 |
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