Ewing, Patricia and Wilke, Andrea and Brockhoff, Gero and Andreesen, Reinhard and Eissner, Guenther and Holler, Ernst and Gerbitz, Armin (2003) Isolation and transplantation of allogeneic pulmonary endothelium derived from GFP transgenic mice. JOURNAL OF IMMUNOLOGICAL METHODS, 283 (1-2). pp. 307-315. ISSN 0022-1759
Full text not available from this repository.Abstract
The isolation of primary endothelial cells from murine tissues has long been a challenge and remains a difficult task. Using GFP transgenic C57/BL6 mice as donors, we describe a reliable method to isolate pulmonary endothelial cells by flow cytometry after staining with DiI-Ac-low density lipoprotein (LDL). After mechanical dissociation of murine lung tissue and enzymatic digestion, adherent cells can be quickly stained and sorted by flow cytometry. The isolated cells express endothelial cell markers such as CD31, MECA32 and CD106 and stained positive for Isolectin B4. After 50-fold expansion using standard endothelial growth media, cells could be transplanted into lethally irradiated allogeneic hosts and were detectable using fluorescence microscopy up to 24-h post-transplantation in pulmonary tissue. (C) 2003 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BONE-MARROW-TRANSPLANTATION; VERSUS-HOST DISEASE; CELLS; endothelium; LDL; cell sorting; lung; mouse; isolation |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Aug 2021 08:34 |
| Last Modified: | 25 Aug 2021 08:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38364 |
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