Lei, Wu and Duerkop, Axel and Lin, Zhihong and Wu, Meng and Wolfbeis, Otto S. (2003) Detection of hydrogen peroxide in river water via a microplate luminescence assay with time-resolved ("gated") detection. MICROCHIMICA ACTA, 143 (4). pp. 269-274. ISSN 1436-5073
Full text not available from this repository.Abstract
Hydrogen peroxide (HP) in river water was determined using the highly luminescent Eu3+-tetracycline hydrogen peroxide (EuTc-HP) complex. A 15-fold increase in luminescence intensity of Eu3+-tetracycline (EuTc) is observed at 616 nm after formation of EuTc-HP. In order to eliminate interferences by other fluorescent substances that exist in river water, a time-resolved scheme is employed: EuTc-HP has a main decay time of 61 mus, while background fluorescence decays within a few ns. Thus, by performing measurements after a delay time of typically 30 mus, the signal becomes highly specific for EuTc-HP. Under optimized conditions (pH 7 in MOPS buffer), the calibration curve for the determination of HP was linear over the range of 2 to 160 mumol L-1. The limit of detection is 1.1 mumol L-1 HP. An average recovery of 99.5% was obtained for measurements of river water samples spiked with HP. The relative standard deviation of less than 2% indicates the high accuracy and precision of the method.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EXCHANGE RESIN; CHEMILUMINESCENCE; OXIDATION; CATALASE; COLUMN; H2O2; hydrogen peroxide; fluorescent probe; europium; tetracycline; time-resolved fluorometry |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Jul 2021 10:41 |
| Last Modified: | 27 Jul 2021 10:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38374 |
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